Fig. 4

Morphologic observation of flowers, fruit and seeds of nontransgenic control plants and VvHB58 overexpressing plants. a Determination of pollen viability and observation of pollen grain and flower morphology. Pollen viability was assessed by FDA staining. NT represents nontransgenic tomatoes. Bars, 100 μm. The morphology of pollen grains was observed using a scanning electron microscope (SEM). Bars, 20 μm. Flowers used in these experiments were collected at full bloom stage. b Morphological change in pericarp due to overexpression of VvHB58. Microscopic cross section through a pericarp of a control fruit and a VvHB58 overexpressing progeny (T1) fruit from representative transformant line OE-8 was used. Bars, 50 μm. c Observation of longitudinal section in seeds between VvHB58 overexpressing progeny and nontransgenic type. Bars, 50 μm