Fig. 1

Real-time quantitative RT-PCR analysis of VvHB58 gene expression levels and subcellular localization of the VvHB58 protein. a Expression levels of VvHB58 during different seed developmental stages from 3-year-old seedless grape cultivars ‘Thompson Seedless’ (T.S) and ‘Flame Seedless’ (F), and seeded grape cultivars ‘Red Globe’ (R.G) and ‘Kyoho’ (K). b Expression levels of the VvHB58 gene during different seed developmental stages from 4-year-old ‘Thompson Seedless’ and ‘Red Globe’. c Expression levels of the VvHB58 gene in different reproductive organs (stamen, pistil, petal and sepal) from 3-year-old seedless cultivar ‘Flame Seedless’ and seeded grape cultivar ‘Muscat Hamburg’. d Expression levels of the VvHB58 gene in different vegetative organs (root, stem, leaf, flower, tendril and fruit) from 3-year-old ‘Flame Seedless’ and ‘Muscat Hamburg’. The grapevine Actin (GenBank Accession number: AY680701) and GAPDH (GenBank Accession number: CB973647) were used as an endogenous control to normalize expression. Bars represent means ± SD from three biological replicates. Different letters indicate statistically significant differences (Dunn’s test; P < 0.05). e Subcellular localization of the VvHB58 protein in tobacco leaves. YFP, yellow fluorescent protein. DAPI, 4,6-diamidino-2-phenylindole dihydrochloride. Bars, 100 μm