Fig. 3
From: Multiplex nucleotide editing by high-fidelity Cas9 variants with improved efficiency in rice
Specificities of high-fidelity Cas9 variants. a Off-target activity detected at three potential off-target sites of ALS, CDC48 and NRT1.1B in the genomics in pBE system. The off-target efficiency was detected using deep sequencing described in method. b Off-target effects of four Cas9 variants detected with guide sequences containing pairs of mismatches at successive positions combined with tRNA-sgRNA and tRNA-modified sgRNA in pBE system. The mismatch sites were highlighted in red with the PAM highlighted in blue. c Off-target activity of SpCas9 and eSpCas9(1.1) detected on two potential off-target sites of GW8-T2 in the genomics using deep sequencing during random mutation