Fig. 1
From: Multiplex nucleotide editing by high-fidelity Cas9 variants with improved efficiency in rice
Comparation of the editing efficiency of rBE and pBE. a Vector structure used for C-T substitution in both rAPOBEC1 base editing (rBE) and PmCDA1 base editing (pBE) system. tRNA-sgRNA architecture used for multiplex editing was also presented. Three or four targets were assembled to test the editing efficiency. b Comparation of C to T substitution efficiency edited by both SpCas9-rBE and SpCas9-pBE system at seven genomic targets guided by wild type sgRNA. For each construction, fifteen transgenic calli were selected independently for Sanger sequencing. Substitution efficiency was defined as the percentage of calli containing C to T conversion at any position in the editing targets