Fig. 7

Transient transformation of the TaGGP coding sequences in N. benthamiana. a Schematic representation of the T-DNAs used for constitutive overexpression of the TaGGP genes in N. benthamiana. RB, right border; 2 x 35S, dual CaMV 35S promoter; TaGGP, coding sequence of TaGGP1-A (1,293 bp), TaGGP1-B (1,293 bp), TaGGP1-D (1,293 bp), TaGGP2-A (1,296 bp), and TaGGP2-B (1,296 bp); nos T, nopaline synthase terminator; 2 x 35S enhanced, dual CaMV 35S promoter enhanced; hptII, hygromycin phosphotransferase II; pA, CaMV poly(A) signal; LB, left border. b Ascorbate concentrations of N. benthamiana leaves co-infiltrated with A. tumefaciens (GV3101 MP90) containing the constructs of interest and a P19 construct to prevent post-transcriptional gene silencing. The control was infiltrated with A. tumefaciens containing the P19 construct alone. The AcGGP gene from kiwifruit driven by a single 35S promoter in the pGreen vector system was used as a positive control. Bars represent mean ± SEM of three infiltrated young leaves. Means that do not share a letter are significantly different (one-way ANOVA followed by Tukey post-hoc test with 95% confidence level)