Fig. 6

Overexpression of GmERF75 in Arabidopsis enhanced tolerance of osmotic stress. a GmERF75-overexpressing lines had longer hypocotyls than the WT and the two erf71 mutants. Arabidopsis seedlings of GmERF75-overexpressing, WT, and mutants grown on MS medium with/without 6% PEG, 75 mM NaCl, or dark condition, respectively. b Overexpression of GmERF75 in Arabidopsis enhance the resistance to salt and drought. Three-week-old seedlings normally grown in soil were supplied with 250 mM NaCl for 2 weeks or were not watered for 1 week and then were re-watered for 1 week. c Hypocotyl length of 35S::GmERF75, erf71, and WT. Fifteen to twenty-five individuals in each treatment were used to count the hypocotyl length. The data was shown as the means ± SD of three biological replicates. d Total chlorophyll content of the mutants, WT (Col-0), and GmERF75 transgenic lines under drought and salt treatment. Three-week-old seedlings of mutants, WT (Col-0), and transgenic lines were supplemented with 250 mM NaCl or un-watered for 1 week, and recovered for 1 week. 0.1 g leaves of each line were collected and used to measure chlorophyll content. The data was shown as the means±SD of five repetitions each lines. Asterisks indicate significant differences from WT at *P < 0.05 and **P < 0.01 determined by Student’s t test