Fig. 5
From: Tomato glycosyltransferase Twi1 plays a role in flavonoid glycosylation and defence against virus
Twi1 gene expression in the Moneymaker and RNAi Twi1 transgenic tomato upon scopoletin, esculetin or umbelliferone treatment. A quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of Twi1 gene expression in the leaves of wt (Moneymaker), and the RNAi Twi1 transgenic tomato lines 1.1 and 28.3 upon the scopoletin (a), esculetin (b) or umbelliferone (c) stem-feeding treatments. The Elongation Factor 1 alpha (eEF1α) gene was used as an endogenous reference. The results correspond to the means±SD of three independent plants from a representative experiment. A t-test was performed with the data from three independent experiments. Asterisks (*) indicate statistical significance with a p value < 0.05 in relation to the wt plants