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Fig. 6 | BMC Plant Biology

Fig. 6

From: Oligo-painting and GISH reveal meiotic chromosome biases and increased meiotic stability in synthetic allotetraploid Cucumis ×hytivus with dysploid parental karyotypes

Fig. 6

Tracking meiotic behavior of individual chromosomes at meiotic metaphase I and anaphase I in S4 generation. a-f The meiotic behavior of C5 H9 and H10 were traced based on C5-oligo probes (red) and 45 s rDNA probes (green). a Three exclusive homologous bivalents of C5, H9 and H10. b Two C5 univalents (red arrows) and two H9 univalents (white arrows). c A homoeologous pairing of C5 and H10 (red arrow) and one H10 univalent (white arrow). d The C5 formed an abnormal bivalent configuration with one C-chromosome with large 45 s rDNA. e The C5 formed an abnormal bivalent configuration with one H-chromosome. The identity of two chromosomes could be diagnosed by combining with Type III probes (red) (insets). f The H9 and H10 were unequal segregation at anaphase I. g-i The meiotic behavior of C7 and H1 were traced based on C7-oligo probes (green) and Type III probes (red). g Two exclusive homologous bivalents of C7 and H1. h Two C7 univalents (red arrows) and two H1 univalents (white arrows). i Two H-chromosomes lagging at anaphase I (white arrows). Bars = 5 μm

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BMC Plant Biology

ISSN: 1471-2229

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