Table 3 Detection of frame shift mutation in varieties with different Al tolerance
Candidate gene | Varieties with different Al tolerance | Position from the initial codon in CDS region | Codon | Amino acid |
|---|---|---|---|---|
LOC_Os01g57350.1 | Nipponbare | 182nd | CAG | Glutamine |
Kasalath | GAG | Glutamic acid | ||
Nipponbare | 261st | TAC | Tyrosine | |
Kasalath | CAC | Histidine | ||
LOC_Os03g30060.1 | Nipponbare | 20th | AAG | Lysine |
Kasalath | CAG | Glutamine | ||
Nipponbare | 66th | GCC | Alanine | |
Kasalath | ACC | Threonine | ||
Nipponbare | 88th | GTG | Valine | |
Kasalath | ATG | Methionine | ||
Nipponbare | 125th | GCA | Alanine | |
Kasalath | GGA | Glycine | ||
Nipponbare | 126th | GCG | Alanine | |
Kasalath | TCG | Serine | ||
Nipponbare | 155th | AAC | Asparagine | |
Kasalath | AGC | Serine | ||
Nipponbare | 184th | GCG | Alanine | |
Kasalath | ACG | Threonine | ||
Nipponbare | 220th | CAG | Glutamine | |
Kasalath | GAG | Glutamic acid | ||
Nipponbare | 246th | GCC | Alanine | |
Kasalath | Deletion | Deletion | ||
Nipponbare | 260th | ATG | Methionine | |
Kasalath | GTG | Valine | ||
Nipponbare | 276th | ATC | Isoleucine | |
Kasalath | GTC | Valine | ||
Nipponbare | 277th | GCG | Alanine | |
Kasalath | ACG | Threonine | ||
LOC_Os07g03050.1 | Nipponbare | 7th | TAT | Tyrosine |
Kasalath | TCT | Serine | ||
Nipponbare | 344th | TGT | Cysteine | |
Kasalath | AGT | Serine | ||
LOC_Os01g57360.1 | Nipponbare | 32nd | AAG | Lysine |
IR64 | AAT | Asparagine | ||
Nipponbare | 348th | GGC | Glycine | |
IR64 | TGC | Cysteine | ||
LOC_Os11g03110.1 | Nipponbare | 261st~269th | Deletion | Deletion |
Kasalath | Insertion | Insertion | ||
Nipponbare | 608th | GTG | Valine | |
Kasalath | GCG | Alanine | ||
LOC_Os01g74200.1 | Nipponbare | 12th | TCG | Cysteine |
IR64 | TCA | Serine | ||
LOC_Os09g33550.1 | Nipponbare | 50th | CAC | Histidine |
IR64 | CCC | Proline | ||
Nipponbare | 58th | GCG | Alanine | |
IR64 | ACG | Threonine | ||
Nipponbare | 65th | GCG | Alanine | |
IR64 | GTG | Valine |