Fig. 5

The proportion of fatty acid compositions in anthers at different developmental stages. a, wild-type anthers; b, fad2–3 anthers; c, the ratio of C18:2 to C18:1. Anthers at different developmental stages was used for fatty acid assay. St1-St5: Anther at different developmental stages; St1, Sporogenous cell stage; St2, Microsporocyte stage; St3, Meiosis stage; St4, Tetrad stage; St5, Pollen maturation stage. The fatty acid methyl esters were prepared by alkaline transmethylation. The analyses were performed using GCMS-QP2020 at an electron ionization of 70 eV with an HP-88 column. The quantification was carried out according to the response value of quantitative ions and the established standard curve. Each test was repeated three times, and the content of each fatty acid composition was calculated as the percentage of total measured fatty acids. The ratio of C18:2/C18:1 is calculated by dividing the relative percentage content of C18:2 from that of C18:1 at the same developmental stage. Each bar represents the mean data of three biological replicates. Error bars are standard errors. Asterisks denote significant differences to wild-type (WT) as determined by Student’s t test: ***p < 0.001