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Table 3 Changes of LN stress on metabolite content in young leaves of two wild soybean varieties

From: Physiological and metabolomics analyses of young and old leaves from wild and cultivated soybean seedlings under low-nitrogen conditions

Metabolite name Relative concentration Fold changes Log2(LN/CK)
C W
CK LN CK LN C W
Aliphatic amino acid Valine 25.53 ± 2.6 5.57 ± 1.11 0.62 ± 0.04 0.54 ± 0.05 −2.20** −0.19**
Isoleucine 15.35 ± 0.21 1.95 ± 0.02 0.39 ± 0.01 0.11 ± 0.00 −2.98** −1.83**
Aspartic acid 14.25 ± 0.13 7.10 ± 0.05 5.30 ± 0.06 9.35 ± 0.01 −1.00 0.82**
Asparagine 48.53 ± 0.19 0.28 ± 0.10 2.58 ± 0.67 0.60 ± 0.76 −7.46** −2.09**
L − homoserine 0.73 ± 0.05 0.13 ± 0.07 0.22 ± 0.00 0.08 ± 0.00 −2.54** −1.56
L − threonine 10.60 ± 0.20 1.17 ± 0.22 1.77 ± 0.03 1.82 ± 0.04 −2.79** 0.04
Serine 47.67 ± 0.04 8.46 ± 0.02 0.19 ± 0.13 0.16 ± 0.25 −2.49** −0.27
L − proline 12.72 ± 0.67 3.47 ± 0.11 0.27 ± 0.00 0.21 ± 0.02 −1.87** −0.40*
Alanine 89.09 ± 0.15 40.72 ± 0.07 0.06 ± 0.04 0.07 ± 0.03 −1.13** 0.35
Glycine 17.12 ± 6.96 2.93 ± 7.54 28.26 ± 0.02 50.61 ± 0.04 −2.55** 0.84*
Glutamate 0.09 ± 0.00 0.03 ± 0.00 0.03 ± 0.00 0.03 ± 0.00 −1.86* −0.31
Aromatic amino acid Tyrosine 3.28 ± 0.11 0.47 ± 0.01 1.68 ± 0.30 1.11 ± 0.04 −2.80** −0.60**
Phenylalanine 4.26 ± 1.44 1.72 ± 0.54 11.31 ± 0.07 10.73 ± 0.02 −1.30** −0.08
Non − protein nitrogen GABA 5.35 ± 0.10 7.28 ± 0.21 0.52 ± 0.01 0.32 ± 0.03 0.44 −0.70*
Ethanolamine 61.53 ± 1.84 24.49 ± 0.50 55.44 ± 0.02 86.12 ± 0.04 −1.33** 0.64*
Mannosamine 0.19 ± 0.31 0.13 ± 0.04 11.32 ± 0.00 7.19 ± 0.01 −0.57* −0.65
Sphingosine 2.49 ± 0.14 2.07 ± 0.06 1.76 ± 0.30 2.34 ± 0.34 −0.27 0.41
Sugars and polyols Sucrose 0.63 ± 0.08 2.08 ± 0.35 0.08 ± 0.07 0.07 ± 0.00 1.72* −0.10
Maltose 1.83 ± 4.23 2.27 ± 0.41 0.33 ± 0.01 0.28 ± 0.02 0.31** −0.21
Maltotriose 0.05 ± 0.02 0.22 ± 0.04 0.93 ± 0.02 0.96 ± 0.03 2.14** 0.04
Melezitose 0.05 ± 0.27 0.28 ± 0.02 28.10 ± 0.38 20.39 ± 0.02 2.54** −0.46
Threitol 0.47 ± 0.02 0.35 ± 0.03 2.57 ± 0.20 1.72 ± 0.08 −0.44** −0.58
Galactinol 0.13 ± 0.02 0.05 ± 0.00 0.71 ± 0.01 0.52 ± 0.02 −1.34* −0.47
Glycerol 12.81 ± 0.07 7.79 ± 0.03 1.91 ± 0.28 1.56 ± 0.27 −0.72* −0.29
Myo − inositol 55.75 ± 0.09 28.6 ± 0.09 0.31 ± 0.02 0.22 ± 0.03 −0.96* −0.48
L − threose 0.42 ± 0.01 0.13 ± 0.00 54.13 ± 0.01 60.66 ± 0.16 −1.68* 0.16
Xylose 0.71 ± 0.01 0.91 ± 0.01 0.09 ± 0.02 0.11 ± 0.01 0.35** 0.29
Glucose−6 − phosphate 0.07 ± 0.00 0.20 ± 0.14 306.86 ± 0.34 216.33 ± 0.17 1.49** −0.50*
Fructose-6 − biphosphate 0.48 ± 0.02 0.14 ± 0.01 0.08 ± 0.00 0.28 ± 0.01 −1.71* 1.84**
Organic acid Glyceric acid 14.14 ± 1.77 6.57 ± 1.27 0.33 ± 0.55 0.43 ± 0.09 −1.11** 0.36
Pyruvic acid 5.89 ± 1.52 2.33 ± 1.10 0.20 ± 0.55 0.13 ± 0.30 −1.34** −0.64*
succinic acid 8.49 ± 0.08 8.19 ± 0.41 0.25 ± 0.02 0.32 ± 0.02 −0.05 0.37*
  Fumaric acid 77.42 ± 0.09 111.54 ± 0.04 4.50 ± 0.25 2.40 ± 0.16 0.53 −0.91*
L − malic acid 0.18 ± 0.32 0.07 ± 0.02 4.04 ± 0.18 3.17 ± 0.15 −1.36** −0.35
Hydroxypropionic acid 0.33 ± 0.29 0.27 ± 0.03 0.02 ± 1.15 0.02 ± 0.58 −0.25 −0.02
Oxalic acid 0.37 ± 2.73 0.28 ± 0.00 0.97 ± 1.79 0.78 ± 0.03 −0.42* −0.31
Itaconic acid 4.36 ± 2.95 3.74 ± 0.00 2.90 ± 0.03 3.91 ± 1.79 −0.22 0.43
Malonate 0.29 ± 9.64 0.08 ± 0.30 4.47 ± 0.08 5.48 ± 0.51 −1.79** 0.30
Maleic acid 9.83 ± 0.15 11.31 ± 0.15 0.08 ± 0.03 0.18 ± 0.03 0.20 1.11*
Gluconic acid 0.56 ± 0.39 0.23 ± 0.57 0.56 ± 0.07 0.78 ± 0.09 −1.31** 0.49**
Nicotinic acid 2.45 ± 0.51 1.73 ± 0.11 4.95 ± 0.58 10.38 ± 0.40 −0.50 1.07**
Threonic acid 2.40 ± 0.34 2.02 ± 0.19 2.23 ± 0.83 0.72 ± 0.00 −0.25 −1.63*
Galactonic acid 1.92 ± 0.62 1.38 ± 0.63 0.83 ± 0.00 0.51 ± 0.10 −0.47* −0.70**
Glucoheptonic acid 0.78 ± 0.26 0.54 ± 0.02 16.23 ± 0.31 8.81 ± 0.41 −0.52 −0.88
Fatty acids Butyrate 0.23 ± 0.05 0.12 ± 0.00 2.73 ± 0.01 1.60 ± 0.40 −0.93** − 0.77
Stearic acid 0.66 ± 0.15 0.29 ± 0.03 0.24 ± 0.01 0.11 ± 0.03 −1.21* −1.18*
Palmitic acid 3.60 ± 0.55 1.28 ± 0.20 42.32 ± 1.77 40.01 ± 1.53 −1.49* −0.08
Linolenic acid 2.55 ± 0.72 1.20 ± 0.17 0.17 ± 0.05 0.14 ± 0.02 −1.09** −0.29
Linoleic acid 0.14 ± 0.01 0.04 ± 0.03 4.13 ± 0.09 2.72 ± 0.13 −1.71** −0.60**
Phenylpropanoids Neohesperidin 0.37 ± 0.00 0.23 ± 0.00 11.03 ± 0.05 7.74 ± 0.21 −0.69* − 0.51*
Salicylic acid 3.97 ± 0.03 1.25 ± 0.13 0.13 ± 0.34 0.18 ± 0.13 −1.66** 0.52
Prunin 0.24 ± 0.09 0.18 ± 0.04 8.47 ± 1.06 13.32 ± 0.36 −0.39 0.65**
Ferulic acid 2.25 ± 0.03 1.09 ± 0.04 13.46 ± 0.03 36.23 ± 0.13 −1.04** 1.43**
Caffeic acid 0.02 ± 0.24 0.01 ± 0.24 1.30 ± 0.00 0.36 ± 0.00 −0.55 −1.86*
  1. Relative concentrations and standard deviation were increased by a factor of 100 times in each treatment. Values were presented as the mean ± standard error of four biological replicates. C Cultivar soybean, W Wild soybean, CK Control treatment, LN Low−N stress; * and ** indicate significant (P < 0.05) and highly significant (P < 0.01) differences, respectively