Fig. 4

Auxin accumulation patterns during LR formation in transgenic tomato plants. Confocal spectral laser scanning microscopy was used to image DR5pro:YFP (green) fluorescence localization during LR development in tomato roots. a LR initiation, during which several cells from pericycle layers have differentiated into a small dome shape. b, c Developing LR primordium. d LR primordium vasculature formation. e Transverse-section of (d) which shows the connection between a developing LR and the primary root. f LR emergence from primary root epidermis. g Two-fold magnification of (f) at the point of emergence from the primary root. h Mature LR. i Primary root. YFP fluorescence signal is shown in green. a, c, d Arrows point to YFP signals in the primary root adjacent to LR primordium. Bars = 100 μm