Table 1 Characterization of AGO, DCL and RDR genes in Citrus sinensis
| Â | Gene Name | Gene ID Phytozome | Gene ID CSAP | Location | Protein | |||
|---|---|---|---|---|---|---|---|---|
ORF length (bp) | Length (AA) | PI | Mw (Da) | |||||
DCL | CsDCL1 | orange1.1g000174m | orange1.1 t00584 | ChrUn: 6828329–6,838,566 (− strand) | 5892 | 1963 | 5.96 | 219,934 |
CsDCL2a | orange1.1g000607m | Cs6g03520 | Chr6: 3969936–3,982,992 (− strand) | 4191 | 1396 | 7.65 | 158,488 | |
CsDCL2b | orange1.1g003062m | Cs6g03500 | Chr6: 3910063–3,922,849 (− strand) | 4206 | 1401 | 6.48 | 158,770 | |
CsDCL3 | orange1.1g000379m | Cs4g06370 | Chr4: 3914566–3,925,649 (+ strand) | 4959 | 1652 | 7.95 | 184,339 | |
CsDCL4 | orange1.1g000380m | Cs4g01340 | Chr4: 255555–267,608 (+ strand) | 4902 | 1633 | 6.47 | 183,256 | |
RDR | CsRDR1a | orange1.1g002586m | Cs2g17570 | Chr2: 14327852–14,333,995 (+ strand) | 3399 | 1132 | 7.13 | 129,642 |
CsRDR1b | orange1.1g003789m | Cs5g14110 | Chr5: 11659292–11,663,430 (− strand) | 3249 | 1082 | 6.04 | 122,608 | |
CsRDR1c | orange1.1g035741m | – | Scaffold00168–67,689 – 71,577 (− strand) | 2910 | 969 | 8.58 | 110,376 | |
CsRDR2 | orange1.1g001183m | Cs5g05170 | Chr5: 3069393–3,074,020 (+ strand) | 3396 | 1131 | 6.59 | 128,958 | |
CsRDR31 | orange1.1g001771m | Cs4g15260 | Chr4: 14278137–14,289,339 (+ strand) | 3048 | 1015 | 6.8 | 115,671 | |
CsRDR6a | orange1.1g041430m | Cs7g05350 | Chr7: 2849984–2,855,672 (+ strand) | 3594 | 1197 | 6.13 | 136,437 | |
CsRDR6b2 | orange1.1g048783m | Cs1g14730 | Chr1: 18022840–18,027,238 (+ strand) | 3501 | 1166 | 6.01 | 132,490 | |
AGO | CsAGO1 | orange1.1g001466m | Cs5g16710 | Chr5: 15851375–15,859,311 (− strand) | 3222 | 1073 | 9.38 | 118,338 |
CsAGO2a | orange1.1g012649m | Cs2g10760 | Chr2: 8058870–8,063,724 (+ strand) | 2946 | 981 | 9.29 | 110,071 | |
CsAGO2b | – | Cs2g10770 | Chr2: 8075960–8,080,634 (+ strand) | 2946 | 981 | 9.26 | 110,304 | |
CsAGO4a | orange1.1g002449m | Cs2g29070 | Chr2: 28612071–28,619,504 (− strand) | 2763 | 920 | 8.98 | 102,998 | |
CsAGO4b | orange1.1g002636m | Cs3g06860 | Chr3: 9639528–9,647,482 (+ strand) | 2697 | 898 | 9.43 | 100,950 | |
CsAGO5a1 | orange1.1g002204m | Cs7g17940 | Chr7: 13746350–13,751,613 (− strand) | 2865 | 954 | 9.27 | 106,773 | |
CsAGO5b2 | orange1.1g003630m | Cs7g17970 | Chr7: 13774473–13,781,129 (− strand) | 2847 | 948 | 9.50 | 105,299 | |
CsAGO5c2 | – | Cs7g17930 | Chr7: 13732122–13,738,824 (− strand) | 3021 | 1006 | 9.28 | 112,004 | |
CsAGO5d | – | Cs6g14430 | Chr6: 15643144–15,650,122 (− strand) | 2688 | 895 | 9.23 | 99,978 | |
CsAGO6 | orange1.1g002661m | Cs2g20520 | Chr2: 17331361–17,339,897 (− strand) | 2688 | 895 | 9.41 | 100,500 | |
CsAGO6-like2 | orange1.1g048669m | Cs6g16080 | Chr6: 17010270–17,016,001 (− strand) | 2814 | 937 | 9.71 | 105,659 | |
CsAGO7 | orange1.1g001684m | Cs7g03360 | Chr7: 1453333–1,457,453 (− strand) | 3093 | 1030 | 9.24 | 117,036 | |
CsAGO10 | orange1.1g001954m | Cs9g07740 | Chr9: 4853982–4,862,787 (− strand) | 2979 | 992 | 9.34 | 111,516 | |