Fig. 2
From: A fern WUSCHEL-RELATED HOMEOBOX gene functions in both gametophyte and sporophyte generations
Expression of CrWOXB in the wild-type sporophyte localized to developing tissues and regions of cellular proliferation. a RT-PCR of CrWOXB expression in sporophytes; 1st, sporophytes with first leaf emerged; WVL, whole vegetative leaf; VLB, vegetative leaf pinna base; VLT, vegetative leaf pinna tip; WS whole sporophyll; SPB, sporophyll pinna base; SPT, sporophytll pinna tip. CrUBQ used as control. b-i In-situ hybridization of sectioned young sporophytes with 10-11vegetative leaves. Black outline depicts boundary of tissues; green arrowheads, leaf apical cell; pink arrowheads, shoot apical cell. b, c Emerging vegetative leaf primordia. d, e Shoot meristem and young leaf primordia. f, g Vascular bundles of older leaf tissues. h, i Root primordia. (b, d, f, h) anti-sense, (c, e, g, i) sense control. Scale bar = 0.1 mm (b-i)