Skip to main content

Table 3 Primer sequences and efficiency of the shortlisted primers used in this study

From: Genome wide search to identify reference genes candidates for gene expression analysis in Gossypium hirsutum

Gene.Name Primers (5′ to 3′) Efficiency (%) R 2
PP2A1 F-GATCCTTGTGGAGGAGTGGA 93.54 0.99
R-GCGAAACAGTTCGACGAGAT   
TMN5 F-CTCACCATTCCATTACTTGTGTTG 103.24 0.97
R-GAGGAATCTCTCTCGGGTATCT   
UBQ14 F-CAACGCTCCATCTTGTCCTT 103.74 0.99
R-TGATCGTCTTTCCCGTAAGC   
TBL6 F-AGCAGATCCAGAGACAAGAAAG 95.14 0.99
R-CCATTGTAGGTGCAGGTGTAT   
UTR5B F-CGGTCTCTGCTGGTTCTTTAG 94.91 0.99
R-TGACATGTTGTGGTTAGGATGT   
At1g65240 F-GCAAACTCTACAGCTCCCATTA 104.42 0.99
R-GTCCAAACCCGAAGATTCCA   
CYP76B6 F-TGGCTTGGATGCCTGTTT 103.71 0.99
R-TCGCCGTAAGTGTTGGTTAG