Fig. 4

DREB1A modulates expression of AtTPPF in vivo. a Schematic illustrating T-DNA location in 5′- untranslated region of AtDREB1A to form dreb1a. Arrows indicate positions of primers used in (b) and (c). b Homozygosity of T-DNA insertion in dreb1a as assessed by PCR analysis of DREB1A genomic fragment using left primer (LP), right primer (RP), and T-DNA-specific left border primer (LB). c DREB1A transcript levels in wild-type (WT) and dreb1a plants as determined by RT-PCR analysis with forward and reverse primers (LP and RP, respectively). eIF-4A served as reference standard. d DREB1A transcript levels in WS and DREB1A-OE (DREB1A-OX) plants as determined by RT-PCR analysis with forward and reverse primers (LP and RP, respectively). eIF-4A served as reference standard. e Relative abundance of AtTPPF transcripts in WT and dreb1a plants under control and drought conditions. Three independent experiments were performed. Error bars indicate SEs (n = 4). Different lowercase letters (a, b or c, d) show significant differences among lines (Duncan’s test; p < 0.05). f Relative abundance of AtTPPF transcripts in WS and DREB1A-OX plants under control and drought conditions. Three independent experiments were performed. Error bars indicate SEs (n = 4). Different lowercase letters (a and b or c and d) indicate significant differences among lines (Duncan’s test; p < 0.05)