Fig. 3

a exon/intron organization of the LjNPF2.9 gene. Insertion sites, couple of primers (black and grey; Additional file 8: Table S4) used for expression analyses and relative orientations of the LORE1 retrotransposon element in the 30,086,034, 30,071,286 and 30,007,925 lines are indicated; b expression analysis of the LjNPF2.9 gene. Total RNAs isolated from root tissues of the wild type and ljnpf2.9 mutants obtained from the three different LORE1 lines has been used for RT-PCR analysis (two different ljnpf2.9–1 mutants, a and b are shown)