Fig. 2

Effects of NO donors on the thermotolerance of cngc6 seedlings. (a) Eight-day-old wild-type and cngc6 seedlings were exposed to 45 °C (HS) or maintained at 22 °C (Control) for 60 min. The NO levels in the plants were then examined by fluorescence microscopy using roots stained with DAF-FM DA. Bar = 100 μm. (b) The relative DAF-FM fluorescence densities in the roots. The data presented are the means ± SE of measurements taken from five independent experiments with at least ten roots for each treatment. *P < 0.05 versus Col. (c) Seedlings were exposed to 45 °C (HS) or maintained at 22 °C (Control) for 100 min, then returned to 22 °C and photographed 5 days later. The clusters are as follows: 1, Col; 2, Col + SNP; 3, Col + SNAP; 4, cngc6; 5, cngc6 + SNP; and 6, cngc6 + SNAP. (d) Survival ratios of the seedlings after HS treatment. The data presented are the means ± SE from at least five independent experiments, with 50 seedlings per experiment. *P < 0.05 versus Col (Student’s t-test)