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Fig. 5 | BMC Plant Biology

Fig. 5

From: Metabolic and transcriptional analyses in response to potent inhibitors establish MEP pathway as major route for camptothecin biosynthesis in Nothapodytes nimmoniana (Graham) Mabb

Fig. 5

HPLC profiling: Chromatograms of authentic standards: camptothecin (a); secologanin (b) and tryptamine (c). HPLC profiles of tissue extracts for detection and quantification of seo-iridoid metabolites, camptothecin (d); secologanin (e) tryptamine (f). The HPLC conditions of secologanin and camptothecin are, acetonitrile: formic acid (99.5:0.5; v/v) delivered at a flow rate of 0.8 mL min− 1. The secologanin and camptothecin were eluted at retention time of 5.6 min and 3.2 min. Respectively. Mobile phase of tryptamine consisted of methanol: water: formic acid (50: 50: 0.1; v/v) at a flow rate of 0.9 mL/min. Tryptamine was eluted at a retention time of 1.7 min

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BMC Plant Biology

ISSN: 1471-2229

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