Fig. 2From: In planta high levels of hydrolysable tannins inhibit peroxidase mediated anthocyanin degradation and maintain abaxially red leaves of Excoecaria CochinchinensisIdentification of the main anthocyanins in the leaves of Excoecaria and Osmanthus, using UPLC-DAD-QTOF-MS/MS. A-B Anthocyanin purification profiles by column chromatography. Absorbance at 510 nm and 280 nm was determined in the fractions of Sephadex LH-20 column chromatography for the anthocyanin purification of Excoecaria (A) and Osmanthus (B) respectively. The major anthocyanin fractions as the indicated peaks between two dotted lines were collected for UPLC-DAD-QTOF-MS/MS analysis. C-D Anthocyanin UPLC-DAD profiles represented by absorbance at 280 nm. The anthocyanin fraction from Excoecaria (C) and Osmanthus (D) leaves as indicated in (A) and (B) were respectively analyzed by UPLC-DAD-QTOF-MS/MS. E-F MS/MS spectra of the main anthocyanins detected in Excoecaria (E) and Osmanthus (F) respectively. The prediction of the anthocyanins in (E-F) were based on the comprehensive MS/MS metabolite database “METLIN” (https://metlin.scripps.edu/)Back to article page