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Fig. 2 | BMC Plant Biology

Fig. 2

From: In planta high levels of hydrolysable tannins inhibit peroxidase mediated anthocyanin degradation and maintain abaxially red leaves of Excoecaria Cochinchinensis

Fig. 2

Identification of the main anthocyanins in the leaves of Excoecaria and Osmanthus, using UPLC-DAD-QTOF-MS/MS. A-B Anthocyanin purification profiles by column chromatography. Absorbance at 510 nm and 280 nm was determined in the fractions of Sephadex LH-20 column chromatography for the anthocyanin purification of Excoecaria (A) and Osmanthus (B) respectively. The major anthocyanin fractions as the indicated peaks between two dotted lines were collected for UPLC-DAD-QTOF-MS/MS analysis. C-D Anthocyanin UPLC-DAD profiles represented by absorbance at 280 nm. The anthocyanin fraction from Excoecaria (C) and Osmanthus (D) leaves as indicated in (A) and (B) were respectively analyzed by UPLC-DAD-QTOF-MS/MS. E-F MS/MS spectra of the main anthocyanins detected in Excoecaria (E) and Osmanthus (F) respectively. The prediction of the anthocyanins in (E-F) were based on the comprehensive MS/MS metabolite database “METLIN” (https://metlin.scripps.edu/)

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