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Table 1 Summary of T0 Fielder-CRISPR regenerated plants expressing Cas9 and their nptII copy number

From: Outlook for coeliac disease patients: towards bread wheat with hypoimmunogenic gluten by gene editing of α- and γ-gliadin gene families

  T0 Fielder wheat plants
Regenerated Expressing Cas9 1 or 2 transgene copies
Construct α1_sgRNA
sgRNA_α87
40 38 (95%) 21 (55%)
Construct α2_sgRNA
sgRNA_α213 + sgRNA_α324
32 12 (38%) 4 (33%)
Construct γ3_sgRNA
sgRNA_γ86 + sgRNA_γ272 + sgRNA_γ603
36 35 (97%) 26 (74%)
Construct α2γ3_sgRNA
sgRNA_α213 + sgRNA_α324 + sgRNA_γ86 + sgRNA_γ272 + sgRNA_γ603
42 32* (76%) 12 (38%)
TOTAL 150 117 (78%) 63 (54%)
  1. For each construct, between 32 and 42 plants were regenerated. The * indicates that 34 plants expressed Cas9 but 2 were discarded since they did not contain all 5 sgRNAs. Overall, 78% of plants expressed the Cas9, among which 54% had 1 or 2 copies of the nptII selectable marker gene whereas the remainder contained additional copies of nptII.