Alignment of α- and γ-gliadin protein sequences with sgRNA position and potential sites of gene editing. A representation of the protein sequence alignments of α-gliadins (a) and γ-gliadins (b) based on a total of 438 and 187 DNA sequences, respectively. The variation in the sequences form patterns which are grouped here and associated to the genome in which they are mostly found (A, B, D on the left side of each group), based on comparison of hexaploid wheat sequences with sequences from diploid relatives. The different gliadin protein domains are indicated and the position of the CD immunogenic epitopes are boxed. The DQ2.5 epitopes box includes the DQ2.5-α1, − α2 and - α3 epitopes (Additional file 1: Figure S1 and Additional file 2: Figure S2). The sgRNAs targeted motifs are highlighted in yellow and the potential gene editing sites are marked with scissors. sgRNA_γ272 may cut multiple times, depending on the number of repetitions of the most abundant γ-gliadin CD epitope, DQ2.5-glia-γ4c, which it targets. Details on the alignment, sequence patterns and CD epitopes can be found in Additional file 1: Figure S1 and Additional file 2: Figure S2. The MEGA and fasta files are also provided as Additional Files.