Fig. 1
From: Disruption of an amino acid transporter LHT1 leads to growth inhibition and low yields in rice
Substrate specificity of OsLHT1. a Representative inward currents induced by amino acids. Oocytes injected with water (upper trace, control) and OsLHT1 cRNA (lower trace) were voltage-clamped at − 70 mV and superfused with different amino acids (10 mM, pH 5.4). b Amino acid transport activity of OsLHT1. Oocytes expressing OsLHT1 were voltage-clamped at − 70 mV and superfused with different amino acids (10 mM, pH 5.4). Substrate-induced currents (background subtracted) were normalized to the Asn-induced current. Data are shown as means ± SD of six independent cells. c Kinetic analysis of Asn transport by OsLHT1. Asn-induced currents were recorded at − 140 mV (holding potential of − 40 mV) in different Asn concentrations. Net Asn-induced currents (background subtracted) were normalized to that obtained in 10 mM Asn, and then fitted to the Michaelis-Menten equation, as shown by the solid line. Data are shown as means ± SD of six independent cells