Fig. 5

Overexpression of FtMYB8 reduces the anthocyanin/PA content in transgenic tobacco plants. a Changes in the floral phenotype in tobacco plants overexpressing FtMYB8. b, c Anthocyanins/PA content in transgenic tobacco leaves. d Detection of flavonoid biosynthetic gene transcriptional levels in FtMYB8 transgenic tobacco leaves. mRNA accumulation of six genes was monitored by qRT-PCR in the WT and transgenic lines. Relative expression was evaluated by the 2^−ΔΔCT method, and gene expression levels were defined as “1” in WT tobacco. Tobacco Ntβ-actin was used as a reference gene. Means were calculated from three repeats, and error bars reflect ±SDs. *P < 0.05