Fig. 4
Analyses of the response of the PFtMYB8 promoter to environmental factors and hormone signals. a-f Histochemical GUS staining of PFtMYB8:GUS seedlings under normal conditions (a, b) or subjected for 5 h to 50 μM MeJA (C), 10 μM ABA (D), UV-B (e) or dark (f). (a-f) Histochemical GUS staining of whole seedlings. (n-1)-(n-3) Histochemical GUS staining of the buds (n-1), roots (n-2) or roots (n-3), where n represents a-f. g Abundance analyses of GUS mRNA in WT and transgenic Arabidopsis whole seedlings. Total RNA was extracted after 5 h of treatment, and each sample contained at least 30 seedlings. Relative expression of the GUS gene was evaluated by the 2-ΔΔCT method, and the accumulation of GUS mRNA in whole transgenic seedlings under normal conditions was defined as “1”. Arabidopsis Atactin2 was used as a reference gene. Means were calculated from three repeats, and error bars reflect ±SDs. ** and * represent extremely significant differences and significant differences, respectively (*P < 0.05, **P < 0.01)