Fig. 5

Semi-qRT-PCR and qRT-PCR analyses of 12 selected PtAPs in various tissues of Populus trichocarpa. a-b Expression of 12 selected PtAP genes using semi-qRT-PCR analysis. Different tissues included phloem (PH), cambium (CA), developing xylem (DX), apical bud (AB), young leaf (YL), mature leaf (ML), petiole (PE) and root (RT). Stem internodes included 1st to 6th, 9th and 12th internodes. c Expression of 12 selected PtAP genes by qRT-PCR analysis. The expression of PtActin2 was used as an internal control. Data are means ± standard error of three technical replicate results. Statistically significant difference between DX and other tissues was determined by t-test. P < 0.05 was marked as * and P < 0.01 was marked as **