Fig. 5

Histological observation of fungi growth in TaAMT2; 3a-knockdown plants challenged by Pst. a Leaves inoculated with Pst were observed at 24, 48 and 120 hpi. The infected sites were observed by fluorescence microscopy. H: haustorium; HMC: haustorial mother cell; SV: substomatal vesicle; IH: infection hyphae. Bar, 50 μm; The number of hyphal branches (b), the number of haustorial mother cells (c), the hyphal length (d) and the infection site areas (e) in TaAMT2; 3a-knockdown plants were compared with that in control plants. The fungal structures were stained with wheat germ agglutinin (WGA) and each result was considered from 30 infection sites. Asterisks indicate significant difference (P < 0.05) from BSMV::γ-inoculated plants using Student’s t-test