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Table 4 Genomic classification, geographic information and genetic diversity analysis of 95 accessions among three Elymus genomes

From: EST-SSR marker development based on RNA-sequencing of E. sibiricus and its application for phylogenetic relationships analysis of seventeen Elymus species

Gen

Species

Accession

SS

Geographic information

Genetic analysis

Origins

La(N)

Lo(E)

Al(m)

Na

Ne

H

I

%P

StH

Elymus sibiricus

PI504462

5

Xining, Qinghai, China (Geo-1)

36.7°

101.8°

2300

1.605

1.358

0.211

0.317

60.5

PI531669

6

Xining, Qinghai, China (Geo-1)

36.6°

101.8°

2400

1.603

1.351

0.208

0.313

60.3

PI499456

6

Shandan, Gansu, China (Geo-1)

38.8°

101.1°

1750

1.652

1.368

0.221

0.334

65.2

PI639859

2

Aba, Sichuan, China (Geo-1)

33.1°

102.6°

3320

1.268

1.189

0.111

0.162

26.8

PI655199

6

Longriba, Sichuan, China (Geo-1)

32.1°

102.6°

3280

1.610

1.322

0.196

0.300

61.0

PI499453

6

Inner Mongolia, China (Geo-2)

43.9°

116.1°

1000

1.580

1.309

0.188

0.287

58.0

PI665507

6

Ulaanbaatar, Mongolia (Geo-2)

48.1°

108.7°

1524

1.600

1.321

0.195

0.297

60.0

PI634231

3

Mongolia (Geo-2)

47.6°

112.4°

747

1.470

1.306

0.179

0.265

47.0

PI610886

6

Dzuunburen, Mongolia (Geo-2)

50.0°

105.7°

920

1.659

1.384

0.226

0.341

65.9

W621576

2

Hatgal, Mongolia (Geo-2)

50.3°

99.8°

2042

1.309

1.219

0.128

0.187

30.9

PI598782

6

Buryat, Russia (Geo-3)

52.2°

109.3°

900

1.596

1.318

0.193

0.295

59.6

PI598789

6

Chita, Russia (Geo-3)

52.1°

113.5°

700

1.635

1.364

0.216

0.327

63.5

PI610991

3

Buryat, Russia (Geo-3)

51.9°

104.8°

630

1.400

1.259

0.151

0.225

40.0

PI655095

6

Bayshint, Mongolia (Geo-4)

49.7°

90.3°

1509

1.685

1.398

0.235

0.354

68.5

PI611014

6

Gorno Altay, Russia (Geo-4)

50.3°

87.7°

950

1.607

1.325

0.196

0.299

60.7

PI634228

6

Gorno Altay, Russia (Geo-4)

51.2°

85.6°

1250

1.573

1.314

0.188

0.287

57.3

PI655084

6

Gorno Altay, Russia (Geo-4)

51.3°

85.6°

1090

1.619

1.341

0.204

0.310

61.9

PI639757

6

Gorno Altay, Russia (Geo-4)

51.7°

85.8°

340

1.591

1.306

0.187

0.287

59.1

PI655140

6

Urumqi, Xinjiang, China (Geo-5)

43.5°

87.0°

1650

1.640

1.383

0.225

0.337

64.0

PI595182

6

Urumqi, Xinjiang, China (Geo-5)

43.9°

87.9°

1250

1.677

1.392

0.233

0.351

67.7

PI595180

4

Urumqi, Xinjiang, China (Geo-5)

43.8°

87.9°

1600

1.509

1.313

0.184

0.275

50.9

PI619577

5

Urumqi, Xinjiang, China (Geo-5)

43.1°

87.6°

2360

1.575

1.351

0.205

0.307

57.5

PI499468

6

Urumqi, Xinjiang, China (Geo-5)

43.7°

87.9°

1770

1.626

1.373

0.220

0.331

62.6

PI499462

2

Urumqi, Xinjiang, China (Geo-5)

43.6°

87.9°

1050

1.268

1.189

0.111

0.162

26.8

PI598780

6

Vladivostok, Russia (Geo-6)

43.2°

132.0°

200

1.552

1.288

0.176

0.270

55.2

PI628699

5

Sovetskaya Gavan, Russia (Geo-6)

49.0°

140.3°

50

1.631

1.338

0.205

0.314

63.1

PI628706

6

Sovetskaya Gavan, Russia (Geo-6)

48.6°

139.9°

40

1.650

1.385

0.227

0.342

65.0

PI598778

6

Kazakhstan (Geo-7)

–

–

–

1.692

1.393

0.233

0.353

69.2

PI659930

5

Kochkorka, Kyrgyzstan (Geo-7)

41.7°

75.7°

2438

1.622

1.376

0.222

0.332

62.2

PI659942

6

Kochkorka, Kyrgyzstan (Geo-7)

41.7°

75.5°

2627

1.687

1.401

0.236

0.356

68.7

 

5.2

    

1.573

1.331

0.197

0.297

57.3

StHY

Elymus cylindricus

WEcy3

9

Yongdeng, Gansu, China

36.7°

103.3°

2300

1.734

1.398

0.239

0.363

73.4

StHY

Elymus dahuricus

WEda5

8

Shandan, Gansu, China

38.8°

101.1°

1750

1.694

1.374

0.225

0.344

69.4

StHY

Elymus excelsus

WEex1

6

Yongdeng, Gansu, China

36.7°

103.3°

2300

1.654

1.364

0.218

0.331

65.4

StHY

Elymus nutans

PI499612

6

Xining, Qinghai, China (Geo-8)

36.7°

101.3°

2450

1.652

1.391

0.229

0.344

65.2

PI619586

6

Dulan, Qinghai, China (Geo-8)

35.8°

97.6°

2950

1.659

1.363

0.218

0.331

65.9

PI531645

6

Qinghai, China (Geo-8)

–

–

–

1.720

1.438

0.255

0.382

72.0

Xiahe15

4

Xiahe, Gansu, China (Geo-8)

35.0°

102°

3100

1.532

1.304

0.184

0.278

53.2

PI639852

5

Xiahe, Gansu, China (Geo-8)

35.1°

102.4°

2960

1.608

1.358

0.212

0.320

60.8

Xiahe48

5

Xiahe, Gansu, China (Geo-8)

34.6°

102.1°

3100

1.650

1.371

0.221

0.335

65.0

PI655186

5

Xiahe, Gansu, China (Geo-8)

35.2°

102.5°

2830

1.617

1.368

0.217

0.326

61.7

PI655193

4

Maqu, Gansu, China (Geo-8)

34.0°

102.1°

3280

1.561

1.339

0.200

0.300

56.1

PI655195

5

Maqu, Gansu, China (Geo-8)

34.0°

102.8°

3350

1.587

1.336

0.201

0.304

58.7

PI655192

5

Maqu, Gansu, China (Geo-8)

34.1°

102.2°

3660

1.656

1.403

0.236

0.353

65.6

Luqu28

5

Luqu, Gansu, China (Geo-8)

34.5°

102.7°

3100

1.593

1.338

0.201

0.304

59.3

PI639855

5

Luqu, Gansu, China (Geo-8)

34.7°

102.5°

3060

1.657

1.406

0.238

0.355

65.7

Luqu27

5

Luqu, Gansu, China (Geo-8)

34.4°

102.5°

3100

1.698

1.406

0.241

0.363

69.8

PI628698

6

Tianzhu, Gansu, China (Geo-8)

37.2°

102.8°

2860

1.694

1.398

0.237

0.357

69.4

PI499451

6

Lanzhou, Gansu, China (Geo-8)

36.1°

103.8°

1520

1.691

1.404

0.238

0.359

69.1

PI499611

4

Lanzhou, Gansu, China (Geo-8)

37.3°

102.8°

2300

1.586

1.363

0.213

0.319

58.6

PI639858

6

Aba, Sichuan, China (Geo-9)

33.1°

102.7°

3460

1.649

1.367

0.218

0.329

64.9

PI619521

6

Luhuo, Sichuan, China (Geo-9)

31.8°

100.5°

3740

1.647

1.375

0.222

0.335

64.7

W622112

4

Luhuo, Sichuan, China (Geo-9)

31.5°

100.5°

3190

1.589

1.375

0.218

0.324

58.9

PI619569

6

Dege, Sichuan, China (Geo-9)

31.9°

99.0°

4110

1.661

1.368

0.221

0.336

66.1

PI619520

6

Barkam, Sichuan, China (Geo-9)

32.0°

102.7°

3300

1.642

1.353

0.213

0.323

64.2

W622107

6

Kangding, Sichuan, China (Geo-9)

30.0°

101.8°

3800

1.673

1.392

0.232

0.350

67.3

PI639862

4

Kangding, Sichuan, China (Geo-9)

30.0°

101.9°

3110

1.516

1.300

0.180

0.272

51.6

W623602

5

Litang, Sichuan, China (Geo-9)

29.9°

100.3°

3780

1.603

1.361

0.212

0.318

60.3

PI619516

3

Sichuan, China (Geo-9)

–

–

–

1.406

1.264

0.154

0.228

40.6

PI619527

6

Lhasa, Tibet, China (Geo-10)

29.7°

91.1°

4460

1.694

1.401

0.238

0.359

69.4

PI619530

6

Lhasa, Tibet, China (Geo-10)

30.0°

90.6°

4020

1.645

1.355

0.212

0.323

64.5

PI619526

6

Gongbogyamda, Tibet, China (Geo-10)

30.0°

93.1°

3600

1.673

1.380

0.228

0.345

67.3

PI619589

6

Nedong, Tibet, China (Geo-10)

29.3°

91.8°

3800

1.656

1.365

0.219

0.332

65.6

PI619533

6

Nedong, Tibet, China (Geo-10)

29.2°

91.8°

3470

1.638

1.347

0.209

0.319

63.8

PI619590

6

Changdu, Tibet, China (Geo-10)

30.7°

97.3°

3740

1.642

1.363

0.218

0.329

64.2

PI619592

4

Changdu, Tibet, China (Geo-10)

31.1°

97.2°

4200

1.511

1.312

0.183

0.275

51.1

PI619525

6

Tibet, China (Geo-10)

31.3°

98.0°

3100

1.647

1.365

0.217

0.329

64.7

PI619522

4

Gongjue, Tibet, China (Geo-10)

30.9°

98.3°

4100

1.551

1.347

0.202

0.301

55.1

PI619532

5

Yangbajain, Tibet, China (Geo-10)

30.1°

90.5°

4150

1.607

1.359

0.212

0.319

60.7

W623617

5

Tibet, China (Geo-10)

30.2°

99.9°

4000

1.624

1.352

0.210

0.319

62.4

PI547394

6

Inner Mongolia, China (Geo-11)

–

–

–

1.645

1.349

0.211

0.322

64.5

PI499450

5

Inner Mongolia, China (Geo-11)

–

–

–

1.668

1.407

0.237

0.355

66.8

PI628675

6

Hotan, Xinjiang, China (Geo-12)

36.3°

80.0°

3300

1.691

1.398

0.236

0.356

69.1

PI619519

6

Burqin, Xinjiang, China (Geo-12)

47.7°

86.9°

450

1.715

1.392

0.236

0.359

71.5

PI619575

5

Habahe, Xinjiang, China (Geo-12)

47.9°

86.2°

1200

1.663

1.392

0.232

0.348

66.3

PI564956

6

Pakistan (Geo-13)

–

–

–

1.684

1.389

0.232

0.350

68.4

W610220

6

Gilgit, Pakistan (Geo-13)

35.0°

74.0°

2736

1.666

1.384

0.228

0.344

66.6

PI406466

6

Russia (Geo-14)

–

–

–

1.671

1.383

0.228

0.344

67.1

StHY

Elymus tangutorum

WEta2

5

Minle, Gansu, China

38.4°

100.8°

2310

1.654

1.394

0.231

0.347

65.4

 

5.5

    

1.637

1.369

0.219

0.330

63.7

StY

Elymus abolinii

PI499585

5

Urumqi, Xinjiang, China

43.8°

88.1°

1900

1.617

1.378

0.220

0.329

61.7

StY

Elymus antiquus

PI564957

2

Nedong, Tibet, China

29.2°

91.8°

3550

1.309

1.219

0.128

0.187

30.9

StY

Elymus burchan-buddae

PI636649

4

Xiahe, Gansu, China

35.2°

102.5°

3000

1.600

1.363

0.214

0.322

60.0

PI655143

7

Barkam, Sichuan, China

32.0°

102.6°

3300

1.720

1.401

0.239

0.363

72.0

PI655210

1

Litang, Sichuan, China

30.2°

99.9°

4000

–

–

–

–

–

StY

Elymus ciliaris

PI531575

7

Beijing, China

39.9°

116.3°

60

1.685

1.387

0.230

0.348

68.5

StY

Elymus gmelinii

PI639761

3

Gorno Altay, Russia

50.6°

86.5°

950

1.460

1.299

0.174

0.259

46.0

PI655100

5

Zhaosu, Xinjiang, China

43.2°

81.2°

2370

1.551

1.320

0.189

0.286

55.1

W621543

2

Erdenebulgan, Miongolia

50.2°

101.1°

1676

1.280

1.198

0.116

0.169

28.0

StY

Elymus longearistatus

PI401282

1

Tehran, Iran

36.1°

51.4°

2850

–

–

–

–

–

StY

Elymus macrochaetus

PI564944

4

Alma Ata, Kazakhstan

43.1°

76.9°

2000

1.509

1.321

0.186

0.278

50.9

StY

Elymus nevskii

PI564925

1

Novosibirsk, Russia

54.8°

83.1°

150

–

–

–

–

–

PI632570

4

Bayan-Olgii, Mongolia

49.0°

89.8°

1848

1.488

1.299

0.175

0.262

48.8

StY

Elymus panormitanus

PI254866

2

Sirsank, Mosul, Iraq

37.6°

44.7°

200

1.315

1.223

0.130

0.190

31.5

StY

Elymus semicostatus

PI564964

3

Pakistan

–

–

–

1.420

1.260

0.155

0.232

42.0

StY

Elymus tibeticus

PI639828

3

Lhasa, Tibet, China

29.3°

91.0°

3500

1.414

1.260

0.154

0.230

41.4

WEti4

8

Tianzhu, Gansu, China

37.0°

103.1°

2400

1.696

1.406

0.239

0.360

69.6

 

3.6

    

1.504

1.309

0.182

0.272

50.4

  1. Gen, Genome; SS, sample size; La, Latitude; Lo, Longitude; Al, Altitude; Na, observed number of alleles; Ne, effective number of alleles; H, Nei’s genetic diversity; I, Shannon’s Information index; %P, the percentage of polymorphic loci; Geo-1 to Geo-7, different populations of Elymus sibiricus with different geographic origins; Geo-8 to Geo-14, different populations of Elymus nutans with different geographic origins