Fig. 3
![Fig. 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12870-019-1812-0/MediaObjects/12870_2019_1812_Fig3_HTML.png)
Regeneration of transgenic tobacco.A: (a) Tobacco plantlets up to 4–5 leaves were selected for infection; (b) Tobacco leaves infected with Agrobacterium tumefaciens were cultured in a sterilized culture medium; (c and e) Budding of tobacco leaves after induction in budding medium; (d) Tobacco that did not transform successfully after being kanamycin-screened died of whiteness; (f-h) Growth of tobacco roots in rooting medium; (b) GUS staining of transgenic tobacco seedlings, pistils, and stamens; (c) Transcript levels of Fm4CL-like 1 in different overexpression lines.