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Fig. 8 | BMC Plant Biology

Fig. 8

From: Genome-wide characterization of a SRO gene family involved in response to biotic and abiotic stresses in banana (Musa spp.)

Fig. 8

MaSRO4-interacting proteins identified by the yeast two-hybrid and Co-IP assays. a Yeast two-hybrid assay of MaSRO4 interacting with MaMYB4 or MaNAC6. AD-MaMYB4 or -MaNAC6 co-transformed with BD empty vector was used as negative controls. The adjacent two clones showed different colonies of each interaction test. b Co-IP assay of MaMYB4 or MaNAC6 interacting with MaSRO4. Genes encoding MaSRO4 tagged with 3HA (MaSRO5-3HA) and MaMYB4 or MaNAC6 tagged with FLAG (MaMYB4- or MaNAC6-FLAG) were co-expressed in Arabidopsis protoplasts. Protein extracts (Input) were immunoprecipitated with anti-FLAG resin. Immunoblots were developed with anti-HA antibody to detect MaSRO4 and with anti-FLAG antibody to detect MaMYB4 and MaNAC6. c Interactions between MaMYB4 and MaSRO4 truncated fragments using the yeast two-hybrid system. Various deletions constructs of MaSRO4 were prepared for domain-domain interaction. The left graphic presented the schematic domains of MaSRO4 and deletion derivatives. Numbers above each truncation indicated the AA coordinates of MaSRO4. The right graph showed the yeast growth (No. 1) and the β-galactosidase assay (No. 2)

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