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Fig. 2 | BMC Plant Biology

Fig. 2

From: Molecular cytogenetic characterisation of Elytrigia ×mucronata, a natural hybrid of E. intermedia and E. repens (Triticeae, Poaceae)

Fig. 2

Mitotic metaphase chromosomes of hexaploid E. ×mucronata after sequential FISH and GISH experiments. a and c GISH in plants 10–1 and 17–4: Aegilops tauschii (digoxigenin, anti-DIG-FITC, yellow pseudocolour), Dasypyrum villosum (biotin, streptavidin-Cy3, blue pseudocolour), Hordeum bogdanii (biotin, streptavidin-Cy3, green pseudocolour), and Pseudoroegneria spicata (Cy5, red). b and d FISH in plants 10–1 and 17–4: 5S rDNA (Cy5, red), 18S rDNA (digoxigenin, anti-DIG-FITC, green), and pSc119.2 (Cy3, white pseudocolour; applied only in 10–1 – b). After FISH, chromosomes were counterstained with DAPI. Structural chromosomal alterations are indicated by numerals (1). Scale bars = 10 μm

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BMC Plant Biology

ISSN: 1471-2229

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