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Fig. 6 | BMC Plant Biology

Fig. 6

From: The Solanum chacoense Fertilization-Related Kinase 3 (ScFRK3) is involved in male and female gametophyte development

Fig. 6

ScFRK3 interacts with the MAP kinase kinase ScMKK3. a. Diagram showing the three different constructs made in pGBKT7 with ScFRK3 as the insert. b. Directed Y2H assays between the three ScFRK3-pBKT7 constructs and the five ScMKKs found in the S. chacoense transcriptome. The only positive interaction is shown between ScFRK3-Cpart and ScMKK3. -LT: Y2H media without leucine and tryptophan; −LTHA/+AbA: Y2H media without leucine, tryptophan, histidine, adenine and with aureobasidin A. c. (i) The interaction between ScFRK3 (35S::ScFRK3-YFPN) and ScMKK3 (35S::ScMKK3-YFPC) is confirmed by BiFC in onion cells. (iii) 35S::ScFRK3-YFPN and 35S::YFPC only (empty vector) used as negative control. (v) 35S::ScMKK3-YFPN and 35S::YFPC only (empty vector) used as negative control. (ii, iv, vi) All BiFC interactions were done in triplicates with at least 10 fluorescent cells per replicate. DIC image of i, iii and v. Scale bars: 50 μm

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