Skip to main content


Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Fig. 5 | BMC Plant Biology

Fig. 5

From: The Solanum chacoense Fertilization-Related Kinase 3 (ScFRK3) is involved in male and female gametophyte development

Fig. 5

Pollen phenotyping in iFRK3 mutants. a. The pollen grains were examined by acetocarmine staining (i-iv), fluorescein diacetate (FDA) staining (v-viii) and scanning microscopy, respectively (ix-xii). For acetocarmine and FDA scale bars: 50 μm. For SEM scale bars: 10 μm. b. Pollen development phenotyping using Hoechst 33342 DNA stain (i, iii, v, vii, ix, xi, xiii, xv, xvii and xix) and acetocarmine staning (ii, iv, vi, viii, x, xii, xiv, xvi, xviii and xx) in WT (i, ii, v, vi, ix, x, xiii, xiv, xvii and xviii) and iFRK3–3 mutant (iii, iv, vii, viii, xi, xii, xv, xvi, xix and xx) plants. PMC stage (i-iv). Tetrad stage (v-viii). Released microspores (ix-xii). Microspores after PMI (xiii-xvi). Mature pollen (xvii-xx). Scale bars: 20 μm

Back to article page