Fig. 3

Quantification analysis of the transiently expressed CiDREB1C in C. intermedia leaves by RT-PCR The CiDREB1C gene expression level in leaves of C. intermedia after infiltrating with pCanG-HA empty vector and CiDREB1C was detected by relative quantitative real-time PCR. The Expression values were calculated using the 2^-ΔΔCT method and CiEF1α was used as reference gene. The error bars represent the means of three technical replicates of each biological replicate ± SD. The experiments were performed two independent biological replicates with similar result. Statistical significance differences from control were determined by Student’s t test (**P < 0.01). The column was made by Graphpad prism 7. C: control