An extracted tetraploid wheat harbouring the BBAA component of common wheat shows anomalous shikimate and sucrose metabolism

Table 1 Differentially expressed proteins (DEP) involved in DNA methylation, lipid metabolism, antioxidase system, and sucrose metabolism among the various wheat genotypes

Gene name	Fold change			Adjusted P value
Gene name	AL/ETW	TAA10/ETW	XX329/ETW	AL/ETW	TAA10/ETW	XX329/ETW
DNA methylation
Methyl-CpG binding domain	0.14	0.72	0.82	0.001	0.812	0.919
Methyl-CpG binding domain 1	0.18	0.96	0.64	0.013	1.000	0.620
Methyl-CpG binding domain 2	0.02	0.02	1.27	0.000	0.000	0.785
Lipid metabolism
Lipoxygenase	0.02	0.02	5.15	0.000	0.000	0.000
Lipid transfer protein	0.14	0.20	0.48	0.001	0.000	0.181
Non-specific lipid-transfer	0.13	0.57	0.42	0.004	0.722	0.179
Non-specific lipid-transfer	0.15	0.09	0.30	0.044	0.000	0.054
Lipoxygenase	0.20	0.80	0.78	0.005	0.923	0.813
Lipoxygenase	0.26	1.74	3.47	0.029	0.125	0.000
Lipoxygenase	0.18	1.13	0.40	0.030	0.929	0.141
Antioxidase system
Peroxidase	0.02	0.02	1.32	0.000	0.000	0.768
Ascorbate peroxidase	0.15	0.44	0.84	0.010	0.315	0.936
Peroxidase	0.09	0.28	0.47	0.000	0.007	0.231
Cu-Zn-SOD	0.25	1.49	2.19	0.026	0.361	0.013
Cu-Zn-SOD	0.18	0.97	1.42	0.001	0.989	0.499
Glutathione peroxidase	0.25	0.76	0.66	0.027	0.838	0.578
Glutathione S-transferase	50.00		50.00	0.000		0.000
Glutathione S-transferase	0.19	1.66	1.14	0.018	0.286	0.823
Glutathione S-transferase	0.24	0.92	0.81	0.018	0.989	0.869
Sugar metabolism
Fructose-1,6-bisphosphatase	0.02	4.26	2.27	0.000	0.000	0.008
Fructose-1,6-bisphosphatase	0.02	4.30	2.57	0.000	0.000	0.005
Fructose-bisphosphate aldolase	0.02	5.70	4.25	0.000	0.017	0.006
Galactose-6-phosphate isomerase	0.02	0.02	0.91	0.000	0.000	0.992
Beta-fructofuranosidase	0.02	0.60	0.70	0.000	0.927	0.882
Glycosyltransferase	0.02	0.02	0.02	0.000	0.000	0.000
Glycosyltransferase	9.03	0.02	10.60	0.000	0.000	0.000
Beta-1,3-glucanase	0.03	0.02	0.06	0.000	0.000	0.000

The wheat genotypes used in this study include: extracted tetraploid wheat (ETW), its common wheat donor (line TAA10, genome BBAADD), a tetraploid wheat line (AL: ALTAR81, genome BBAA), and a resynthesized allohexaploid wheat (XX329, genome BBAADD) obtained by crossing ETW (maternal parent) and TQ18 (paternal parent). Only those proteins that showed at least a twofold difference were considered. Adjusted P values < 0.05. Only DEPs between ETW and AL are listed. Each genotype had four biological replicates, and each biological replicate comprised a pool of five plants

ISSN: 1471-2229