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Table 3 Details of the identified 2-DE-resolved exclusive DEPs by LC MS/MS in the leaves of tolerant (Flip 97-43c; black columns) versus susceptible (Flip 97-196c; gray columns) chickpea seedlings treated with 100 mM NaCl stress for 1, 3, 6, and 10 days. The key potential proteins contributing to the differential response of the two genotypes to salinity are indicated by * next to their spot numbers

From: Comparative proteomics and gene expression analyses revealed responsive proteins and mechanisms for salt tolerance in chickpea genotypes

Spot no.

Identified protein

Ac. No. a

Species

MASCOT score

% Coverage (matches) b

TMr/ EMr c

TpI/ EpI d

Time kinetics (DAT)e

1

3

6

10

 

1. Photosynthesis and bioenergy

3*

RuBisCO large subunit-binding protein subunit beta

XP_012567814.1

Cicer arietinum

1137

44 (93)

62.9/59.2

5.85/5.53

70

RuBisCO large subunit, partial (chloroplast) 2

ALB07273.1

Acanthus ebracteatus

71

32 (15)

14.4/17.5

9.39/5.37

84

Ribulose bisphosphate carboxylase small chain

Q9ZP07

Cicer arietinum

270

29 (64)

20.4/9.25

9.03/6.72

18

ATP synthase beta subunit, partial (chloroplast)

AAK72753.1

Cucurbita pepo

925

38 (128)

51.9/45.2

5.04/5.76

33

ATP synthase subunit beta, chloroplastic

B5LMK9

Cicer arietinum

585

39 (58)

52.9/28.7

5.16/4.99

53*

ATP synthase subunit alpha

A0A023HPK1

Trifolium aureum

3840

27 (149)

55.8/24.1

5.16/5.33

49*

psbP domain-containing protein 1, chloroplastic

XP_004494530.1

Cicer arietinum

643

65 (31)

28.6/20.5

8.89/5.79

52*

Chlorophyll a-b binding protein 3, chloroplastic

XP_004491629.1

Cicer arietinum

244

25 (55)

29.4/21.3

3.32/5.34

64*

Oxygen-evolving enhancer protein 1, chloroplastic

XP_004509219.1

Cicer arietinum

214

35 (37)

34.9/19.0

6.24/4.72

79

Oxygen-evolving enhancer protein 2, chloroplastic

XP_004499534.1

Cicer arietinum

537

32 (60)

28.7/16.5

6.90/5.90

74

Photosystem I reaction center subunit II, chloroplastic-like

XP_004511355.1

Cicer arietinum

625

34 (37)

23.7/12.5

9.66/5.00

68

2Fe-2S iron-sulfur cluster-binding domain protein

G7IUN0

Medicago truncatula

70

8 (1)

21.5/15.7

6.82/5.11

69

Cytochrome b6-f complex iron-sulfur subunit

A0A067FP73

Gossypium raimondii

61

13 (4)

32.6/16.7

8.48/5.16

27

Magnesium-chelatase subunit ChlI, chloroplastic-like

XP_004485870.1

Cicer arietinum

244

34 (32)

45.4/39.5

5.32/5.06

26

Phosphoribulokinase, chloroplastic

XP_004512451.1

Cicer arietinum

369

30 (28)

45.5/38.8

6.41/5.24

45*

Carbonic anhydrase, chloroplastic isoform X2

XP_004489275.1

Cicer arietinum

409

19 (36)

35.8/22.3

6.61/6.66

38*

Fructose-bisphosphate aldolase 1, chloroplastic

XP_004507507.1

Cicer arietinum

1376

21 (68)

43.2/33.5

6.28/5.98

63

Phosphoglycerate kinase, cytosolic-like

XP_004488763.1

Trifolium aureum

1452

35 (121)

49.8/19.2

7.79/4.66

78

Transketolase, chloroplastic

XP_012570278.1

Cicer arietinum

605

18 (25)

79.9/13.5

6.00/5.95

 

2. Photorespiration

7

Glycine dehydrogenase/decarboxylating, mitochondrial

XP_004498896.2

Cicer arietinum

653

26 (42)

115.3/60.2

7.99/6.43

21

Glutamate--glyoxylate aminotransferase

XP_004489787.1

Cicer arietinum

359

33 (28)

53.3/35.7

5.83/5.88

28*

D-glycerate 3-kinase, chloroplastic

XP_004486714.1

Cicer arietinum

116

19 (12)

46.4/37.2

5.39/5.11

 

3. Stress responsive

1

70 kDa heat shock protein

Q1SKX2

Medicago truncatula

153

11 (12)

75.7/64.7

5.19/4.96

4*

Heat shock 70 kDa protein, mitochondrial isoform

XP_012572445.1

Cicer arietinum

833

40 (67)

72.1/64.7

5.70/5.54

11

Heat shock protein 70 kDa

A0A0A1HAD2

Chrysanthemum morifolium

265

13 (55)

70.8/42.2

5.12/4.86

58

Heat shock 22 kDa protein, mitochondrial isoform X1

XP_004506342.1

Cicer arietinum

249

25 (22)

25.1/18.75

6.78/5.11

81

Cold shock protein

A0A088FZS5

Cicer arietinum

1497

31 (51)

19.2/18.5

6.29/6.55

48*

20 kDa chaperonin, chloroplastic-like

XP_004508023.1

Cicer arietinum

413

50 (17)

26.7/21.5

9.05/6.63

30*

uncharacterized protein, Homologues with LEA-2

XP_004513216.1

Cicer arietinum

400

59 (49)

34.3/35.0

4.63/ 4.76

31

Thiamine thiazole synthase, chloroplastic

I3TAU0

Lotus japonicus

133

17 (12)

37.7/33.2

5.02/5.24

40

Xanthoxin dehydrogenase

XP_004499827.1

Cicer arietinum

573

41 (49)

29.6/28.3

5.89/ 6.19

42*

Glutathione s-transferase

A0A0X9LEN0

Cicer arietinum

270

34 (38)

25.6/21.8

6.04/6.12

41*

L-ascorbate peroxidase, cytosolic

XP_004505943.1

Cicer arietinum

243

37 (49)

27.1/23.2

5.65/ 5.94

55

L-ascorbate peroxidase, cytosolic

XP_004505943.1

Cicer arietinum

501

81 (67)

27.1/21.5

5.65/5.10

77

Superoxide dismutase [Cu-Zn]

A0A0V0HK97

Solanum chacoense

1057

15 (72)

22.4/14.5

6.08/5.96

83

Apolipoprotein D-like

XP_004507714.1

Cicer arietinum

128

35 (29)

21.3/16.5

6.84/6.73

 

4. Protein synthesis and degradation

2*

ATP-dependent zinc metalloprotease FTSH 2

XP_004504668.1

Cicer arietinum

945

36 (74)

74.6/62.7

5.60/5.34

6

Probable mitochondrial-processing peptidase subunit beta

XP_004492264.1

Cicer arietinum

520

41 (56)

59.4/54.5

6.22/6.18

60

Metacaspase-4-like

XP_004510001.1

Cicer arietinum

158

10 (14)

45.2/22.0

5.08/4.76

25

26S protease regulatory subunit 6A homolog

XP_004495708.1

Cicer arietinum

382

50 (29)

47.4/47.0

4.98/5.20

43

Proteasome subunit beta type

A0A151TBQ9

Cajanus cajan

68

21 (10)

24.6/21.5

6.89/6.27

13

Elongation factor Tu, mitochondrial

XP_004493639.2

Cicer arietinum

272

30 (54)

49.1/40.2

6.58/6.44

20

Elongation factor Tu, chloroplastic

XP_004501869.1

Cicer arietinum

388

30 (33)

53.5/44.0

6.25/5.87

23*

Elongation factor Tu, chloroplastic

XP_004501869.1

Cicer arietinum

562

29 (52)

53.5/39.2

6.25/5.31

24

30S ribosomal protein S1, chloroplastic

XP_004486520.1

Cicer arietinum

721

64 (48)

44.5/40.7

5.33/5.18

72

60S acidic ribosomal protein P3-like

XP_004504130.1

Cicer arietinum

420

38 (30)

12.1/14.2

4.20/4.23

85

50S ribosomal protein L9, chloroplastic

XP_004506080.1

Cicer arietinum

27

9 (3)

22.4/15.7

9.78/4.6

16*

peptidyl-prolyl cis-trans isomerase CYP38, chloroplastic

XP_004489294.1

Cicer arietinum

628

43 (68)

50.1/38.5

5.12/4.76

 

5. Amino acid and nitrogen metabolism

19

S-adenosylmethionine synthase

C3TS15

Cicer arietinum

462

50 (61)

43.3/43.7

5.50/5.81

35

Glutamine synthetase leaf isozyme, chloroplastic

Q9XQ94

Medicago sativa

65

14 (30)

47.1/33.0

6.29/5.42

39*

Glycerate dehydrogenase

XP_004497175.1

Cicer arietinum

963

36 (80)

42.2/39.2

6.62/6.95

46

Hydroxyacylglutathione hydrolase 2, mitochondrial-like isoform X

XP_004486896.1

Cicer arietinum

20

5 (2)

36.7/21.3

9.03/6.87

 

6. Other metabolisms (TCA, PP, and purine)

12

Isocitrate dehydrogenase [NADP]

G7KFV7

Medicago truncatula

137

14 (39)

45.9/42.7

5.99/6.43

15

6-phosphogluconate dehydrogenase, decarboxylating 3

XP_004491970.2

Cicer arietinum

524

43 (77)

53.6/47.2

5.88/5.99

36

Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic/mitochondrial-like

XP_004500242.1

Cicer arietinum

131

18 (14)

41.1/29.7

5.65/5.06

 

7. Signaling

47*

Auxin-binding protein ABP19a-like

XP_004513480.1

Cicer arietinum

569

28 (46)

21.9/20.7

6.95/6.91

80*

Low molecular weight phosphotyrosine protein phosphatase

XP_004506147.1

Cicer arietinum

287

12 (12)

27.1/17.7

7.62/5.9

 

8. Gene transcription and replication

82

Nascent polypeptide-associated complex subunit beta

B7FMW7

Medicago truncatula

327

42 (24)

17.5/17.5

6.75/6.83

14

Polyadenylate-binding protein RBP45B isoform X2

XP_012573405.1

Cicer arietinum

277

16 (14)

34.8/44.7

6.31/6.46

37*

33 kDa ribonucleoprotein, chloroplastic

A0A0B2SU82

Glycine soja

66

3 (2)

30.4/31.5

8.67/4.98

54*

29 kDa ribonucleoprotein A, chloroplastic

XP_004497514.1

Cicer arietinum

110

16 (5)

30.7/23.2

5.36/4.83

65

LOC101493535 isoform X1, homologous with smad/FHA domain protein

XP_004502003.1

Cicer arietinum

1469

35 (93)

24.3/16.5

5.69/4.78

71

Histone H2B

A0A0K9PL95

Zostera marina

109

20 (18)

15.5/15.3

10.05/5.3

75

Glycine-rich RNA-binding protein-like

XP_004507449.1

Cicer arietinum

252

22 (27)

16.1/13.5

6.32/5.43

  1. aAccession numbers correspond to NCBI or Uniprot entries
  2. bPercentage of the protein sequence covered by matching peptides and number of matched peptides in the database
  3. cTheoretical molecular weight (Mr) and pI, predicted from MS/MS analysis
  4. dExperimental molecular weight (Mr) and pI were estimated using standard protein markers and automatic assignment by image analyzer (Image Master) software
  5. eProtein expression profile of T1 (dark columns) and S2 (light columns) genotype relative to control, shown as horizontal axis
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BMC Plant Biology

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