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Table 3 Details of the identified 2-DE-resolved exclusive DEPs by LC MS/MS in the leaves of tolerant (Flip 97-43c; black columns) versus susceptible (Flip 97-196c; gray columns) chickpea seedlings treated with 100 mM NaCl stress for 1, 3, 6, and 10 days. The key potential proteins contributing to the differential response of the two genotypes to salinity are indicated by * next to their spot numbers

From: Comparative proteomics and gene expression analyses revealed responsive proteins and mechanisms for salt tolerance in chickpea genotypes

Spot no. Identified protein Ac. No. a Species MASCOT score % Coverage (matches) b TMr/ EMr c TpI/ EpI d Time kinetics (DAT)e
1 3 6 10
  1. Photosynthesis and bioenergy
3* RuBisCO large subunit-binding protein subunit beta XP_012567814.1 Cicer arietinum 1137 44 (93) 62.9/59.2 5.85/5.53
70 RuBisCO large subunit, partial (chloroplast) 2 ALB07273.1 Acanthus ebracteatus 71 32 (15) 14.4/17.5 9.39/5.37
84 Ribulose bisphosphate carboxylase small chain Q9ZP07 Cicer arietinum 270 29 (64) 20.4/9.25 9.03/6.72
18 ATP synthase beta subunit, partial (chloroplast) AAK72753.1 Cucurbita pepo 925 38 (128) 51.9/45.2 5.04/5.76
33 ATP synthase subunit beta, chloroplastic B5LMK9 Cicer arietinum 585 39 (58) 52.9/28.7 5.16/4.99
53* ATP synthase subunit alpha A0A023HPK1 Trifolium aureum 3840 27 (149) 55.8/24.1 5.16/5.33
49* psbP domain-containing protein 1, chloroplastic XP_004494530.1 Cicer arietinum 643 65 (31) 28.6/20.5 8.89/5.79
52* Chlorophyll a-b binding protein 3, chloroplastic XP_004491629.1 Cicer arietinum 244 25 (55) 29.4/21.3 3.32/5.34
64* Oxygen-evolving enhancer protein 1, chloroplastic XP_004509219.1 Cicer arietinum 214 35 (37) 34.9/19.0 6.24/4.72
79 Oxygen-evolving enhancer protein 2, chloroplastic XP_004499534.1 Cicer arietinum 537 32 (60) 28.7/16.5 6.90/5.90
74 Photosystem I reaction center subunit II, chloroplastic-like XP_004511355.1 Cicer arietinum 625 34 (37) 23.7/12.5 9.66/5.00
68 2Fe-2S iron-sulfur cluster-binding domain protein G7IUN0 Medicago truncatula 70 8 (1) 21.5/15.7 6.82/5.11
69 Cytochrome b6-f complex iron-sulfur subunit A0A067FP73 Gossypium raimondii 61 13 (4) 32.6/16.7 8.48/5.16
27 Magnesium-chelatase subunit ChlI, chloroplastic-like XP_004485870.1 Cicer arietinum 244 34 (32) 45.4/39.5 5.32/5.06
26 Phosphoribulokinase, chloroplastic XP_004512451.1 Cicer arietinum 369 30 (28) 45.5/38.8 6.41/5.24
45* Carbonic anhydrase, chloroplastic isoform X2 XP_004489275.1 Cicer arietinum 409 19 (36) 35.8/22.3 6.61/6.66
38* Fructose-bisphosphate aldolase 1, chloroplastic XP_004507507.1 Cicer arietinum 1376 21 (68) 43.2/33.5 6.28/5.98
63 Phosphoglycerate kinase, cytosolic-like XP_004488763.1 Trifolium aureum 1452 35 (121) 49.8/19.2 7.79/4.66
78 Transketolase, chloroplastic XP_012570278.1 Cicer arietinum 605 18 (25) 79.9/13.5 6.00/5.95
  2. Photorespiration
7 Glycine dehydrogenase/decarboxylating, mitochondrial XP_004498896.2 Cicer arietinum 653 26 (42) 115.3/60.2 7.99/6.43
21 Glutamate--glyoxylate aminotransferase XP_004489787.1 Cicer arietinum 359 33 (28) 53.3/35.7 5.83/5.88
28* D-glycerate 3-kinase, chloroplastic XP_004486714.1 Cicer arietinum 116 19 (12) 46.4/37.2 5.39/5.11
  3. Stress responsive
1 70 kDa heat shock protein Q1SKX2 Medicago truncatula 153 11 (12) 75.7/64.7 5.19/4.96
4* Heat shock 70 kDa protein, mitochondrial isoform XP_012572445.1 Cicer arietinum 833 40 (67) 72.1/64.7 5.70/5.54
11 Heat shock protein 70 kDa A0A0A1HAD2 Chrysanthemum morifolium 265 13 (55) 70.8/42.2 5.12/4.86
58 Heat shock 22 kDa protein, mitochondrial isoform X1 XP_004506342.1 Cicer arietinum 249 25 (22) 25.1/18.75 6.78/5.11
81 Cold shock protein A0A088FZS5 Cicer arietinum 1497 31 (51) 19.2/18.5 6.29/6.55
48* 20 kDa chaperonin, chloroplastic-like XP_004508023.1 Cicer arietinum 413 50 (17) 26.7/21.5 9.05/6.63
30* uncharacterized protein, Homologues with LEA-2 XP_004513216.1 Cicer arietinum 400 59 (49) 34.3/35.0 4.63/ 4.76
31 Thiamine thiazole synthase, chloroplastic I3TAU0 Lotus japonicus 133 17 (12) 37.7/33.2 5.02/5.24
40 Xanthoxin dehydrogenase XP_004499827.1 Cicer arietinum 573 41 (49) 29.6/28.3 5.89/ 6.19
42* Glutathione s-transferase A0A0X9LEN0 Cicer arietinum 270 34 (38) 25.6/21.8 6.04/6.12
41* L-ascorbate peroxidase, cytosolic XP_004505943.1 Cicer arietinum 243 37 (49) 27.1/23.2 5.65/ 5.94
55 L-ascorbate peroxidase, cytosolic XP_004505943.1 Cicer arietinum 501 81 (67) 27.1/21.5 5.65/5.10
77 Superoxide dismutase [Cu-Zn] A0A0V0HK97 Solanum chacoense 1057 15 (72) 22.4/14.5 6.08/5.96
83 Apolipoprotein D-like XP_004507714.1 Cicer arietinum 128 35 (29) 21.3/16.5 6.84/6.73
  4. Protein synthesis and degradation
2* ATP-dependent zinc metalloprotease FTSH 2 XP_004504668.1 Cicer arietinum 945 36 (74) 74.6/62.7 5.60/5.34
6 Probable mitochondrial-processing peptidase subunit beta XP_004492264.1 Cicer arietinum 520 41 (56) 59.4/54.5 6.22/6.18
60 Metacaspase-4-like XP_004510001.1 Cicer arietinum 158 10 (14) 45.2/22.0 5.08/4.76
25 26S protease regulatory subunit 6A homolog XP_004495708.1 Cicer arietinum 382 50 (29) 47.4/47.0 4.98/5.20
43 Proteasome subunit beta type A0A151TBQ9 Cajanus cajan 68 21 (10) 24.6/21.5 6.89/6.27
13 Elongation factor Tu, mitochondrial XP_004493639.2 Cicer arietinum 272 30 (54) 49.1/40.2 6.58/6.44
20 Elongation factor Tu, chloroplastic XP_004501869.1 Cicer arietinum 388 30 (33) 53.5/44.0 6.25/5.87
23* Elongation factor Tu, chloroplastic XP_004501869.1 Cicer arietinum 562 29 (52) 53.5/39.2 6.25/5.31
24 30S ribosomal protein S1, chloroplastic XP_004486520.1 Cicer arietinum 721 64 (48) 44.5/40.7 5.33/5.18
72 60S acidic ribosomal protein P3-like XP_004504130.1 Cicer arietinum 420 38 (30) 12.1/14.2 4.20/4.23
85 50S ribosomal protein L9, chloroplastic XP_004506080.1 Cicer arietinum 27 9 (3) 22.4/15.7 9.78/4.6
16* peptidyl-prolyl cis-trans isomerase CYP38, chloroplastic XP_004489294.1 Cicer arietinum 628 43 (68) 50.1/38.5 5.12/4.76
  5. Amino acid and nitrogen metabolism
19 S-adenosylmethionine synthase C3TS15 Cicer arietinum 462 50 (61) 43.3/43.7 5.50/5.81
35 Glutamine synthetase leaf isozyme, chloroplastic Q9XQ94 Medicago sativa 65 14 (30) 47.1/33.0 6.29/5.42
39* Glycerate dehydrogenase XP_004497175.1 Cicer arietinum 963 36 (80) 42.2/39.2 6.62/6.95
46 Hydroxyacylglutathione hydrolase 2, mitochondrial-like isoform X XP_004486896.1 Cicer arietinum 20 5 (2) 36.7/21.3 9.03/6.87
  6. Other metabolisms (TCA, PP, and purine)
12 Isocitrate dehydrogenase [NADP] G7KFV7 Medicago truncatula 137 14 (39) 45.9/42.7 5.99/6.43
15 6-phosphogluconate dehydrogenase, decarboxylating 3 XP_004491970.2 Cicer arietinum 524 43 (77) 53.6/47.2 5.88/5.99
36 Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic/mitochondrial-like XP_004500242.1 Cicer arietinum 131 18 (14) 41.1/29.7 5.65/5.06
  7. Signaling
47* Auxin-binding protein ABP19a-like XP_004513480.1 Cicer arietinum 569 28 (46) 21.9/20.7 6.95/6.91
80* Low molecular weight phosphotyrosine protein phosphatase XP_004506147.1 Cicer arietinum 287 12 (12) 27.1/17.7 7.62/5.9
  8. Gene transcription and replication
82 Nascent polypeptide-associated complex subunit beta B7FMW7 Medicago truncatula 327 42 (24) 17.5/17.5 6.75/6.83
14 Polyadenylate-binding protein RBP45B isoform X2 XP_012573405.1 Cicer arietinum 277 16 (14) 34.8/44.7 6.31/6.46
37* 33 kDa ribonucleoprotein, chloroplastic A0A0B2SU82 Glycine soja 66 3 (2) 30.4/31.5 8.67/4.98
54* 29 kDa ribonucleoprotein A, chloroplastic XP_004497514.1 Cicer arietinum 110 16 (5) 30.7/23.2 5.36/4.83
65 LOC101493535 isoform X1, homologous with smad/FHA domain protein XP_004502003.1 Cicer arietinum 1469 35 (93) 24.3/16.5 5.69/4.78
71 Histone H2B A0A0K9PL95 Zostera marina 109 20 (18) 15.5/15.3 10.05/5.3
75 Glycine-rich RNA-binding protein-like XP_004507449.1 Cicer arietinum 252 22 (27) 16.1/13.5 6.32/5.43
  1. aAccession numbers correspond to NCBI or Uniprot entries
  2. bPercentage of the protein sequence covered by matching peptides and number of matched peptides in the database
  3. cTheoretical molecular weight (Mr) and pI, predicted from MS/MS analysis
  4. dExperimental molecular weight (Mr) and pI were estimated using standard protein markers and automatic assignment by image analyzer (Image Master) software
  5. eProtein expression profile of T1 (dark columns) and S2 (light columns) genotype relative to control, shown as horizontal axis