Fig. 4

Localization of repetitive sequences on A. sativa metaphase chromosomes by in situ hybridization. Root tip chromosomes fluoresce cyan with DAPI (UV excitation) stain; probe hybridization sites are detected by fluorescence shown in red, green and/or blue (false colour from near-infra-red fluorescence). (A-C) C-genome specific probes; (D-E) D-genome specific probes. a Probes AF226603_45bp (red), Ast-R87 (green; yellow where probes overlapping) and pAs120a (blue) from A. brevis. b Probes As_16mer43bp (red) and Ab-T145 (green). (c-e) Metaphases (right) and chromosomes arranged as karyotypes showing genome origin (left) with chromosomes approximately paired by probe pattern and size (Chromosomes 1–14 C-genome; 15–28 A-genome; 29–42 D-genome). White asterisks, arrows, and arrowheads indicate notable C-, A-, and D-chromosome signals. c Probes AF226603_45bp (red), As-T153 (amplified from A. eriantha; green) and pAs120a (blue). d Probes AF226603_45bp (red), Ast-R171 (green), and pAs120a (blue) both amplified from A. strigosa. e Probes AF226603_45bp (red), Ast-T116 (green), and pAs120a (blue). Scale bars = 5 μm