Fig. 8
Quantitative RT-PCR analysis of expression of BnPAP2 and BnPAP15 in BnPHT1;4 overexpression transgenic Brassica napus. (a and b) Expression of BnPAP2 and BnPAP15 (a) and BnACP5, BnIPS1, BnRNS1 and BnPHO1 (b) in 9-h-imbibed seeds of the BnPHT1;4 overexpression transgenic lines and controls (WT and CK) on 1/2 MS medium with 1000 μM Pi. (c) BCIP staining assay of phosphatase activity in 9-h-imbibed seeds of the BnPHT1;4 overexpression transgenic lines and controls (WT and CK) on 1/2 MS medium with 1000 μM Pi. (d) Expression of BnPAP2 and BnPAP15 in cotyledons of oilseed rape seedlings with different Pi treatments. The seven-day-old WT seedlings were transferred into liquid medium with 5, 20, 50, 100, 400, 1000 and 2000 μM Pi for 2 days, and then total RNA was isolated from cotyledons for RT-PCR analysis, using BnACT2 gene as standard control. KCl was used to replace KH2PO4 in the medium for the equivalent amount of potassium. The error bars indicate the standard errors. Significance of difference was analyzed by Duncan’s test (*p<0.05, **p<0.01, n = 3) in a and b, and different lowercase letters indicate statistically significant differences in d (P < 0.05, n = 3)