Fig. 2

The cloning strategy for the WCSV-1 full-length genome. a A linear genomic map of WCSV-1 is shown, in which the putative ORFs are represented by rectangles; b the 12 contigs (H1-H12) obtained from small RNA sequencing are presented as gray lines (B1), and the four fragments (labeled A-D) amplified by the first PCR reaction using primers based on the contigs are indicated by black lines (B2); c three fragments e–g amplified by the second PCR using primers from the obtained clones to fill in the gaps are shown as double arrows. The relative positions of primers are also indicated