Table 2 Novel SSR markers developed in this study from apple contigs
Marker | Primer sequence (5′ → 3′) | Origin of apple contig | Contig start position (bp) | SSR start position in contig (bp) | Motif type | Copy number |
|---|---|---|---|---|---|---|
Mdo.chr11.33 m | F: GTTCGATCGGGGTGAAAGT R: CCCCATCCATTTACCCTACC | MDC021160.220 | 3,150,224 | 6672 | GA | 15.5 |
3_34–12 | F: CCAATTGAAGACCTCCCAAA R: CCAGGAAAAGGACGCTACTG | MDC004702.449 | 3,152,413 | 4269 | TAA | 5.7 |
3_34–18 | F: GAATCCCGAACTGAACCAAA R: GCTAAAATTTGGGCTTTAGGC | MDC001844.206 | 3,160,267 | 223 | AT | 13 |