Fig. 1

Screening in vitro of mutants altered in leaf development. a 12-day-old seedling of tomato cv Moneymaker. b Seedling of 2489-MM (bst) mutant showing curly cotyledons, thicker hypocotyls and enhanced root system. c Seedling of 1527-MM (wl-1527) showing shoestring leaves. d Shoot-derived plants of 150-P73 (wl-150) also develop shoestring leaves. e Shoot apex-derived plants of the mutant 4196-SP (Pwm) show extremely curly leaves and develop a profuse root system. f Shoot-derived plants of Moneymaker (left) and mutant 2733-MM (right). Note the smaller size of the leaves and the shorter internodes of the mutant. g Plants of the mutant 2059-MM exhibit a smaller leaf size and a less developed root system. h Architecture of the fourth leaf of an axenic plant of the tomato cv P73. i The mutant 282-P73 (Art) was identified in vitro due to the irregular arrangement and reduced width of leaflets. j Architecture of the fourth leaf of an axenic plant of the mutant 605-P73. k Leaflet morphology in plants grown in vitro of tomato cv P73 (left) and mutant 605-P73 (right). l The leaves of mutant 744-P73 (Rgb) are smaller and show a slight or moderate bending. m In the mutant 1491-MM (Jmr), leaf wrinkling is particularly intense in the first leaves of shoot apex- and nodal explants-derived plants. n The leaf wrinkling in the mutant 2742-MM (wao) is easily observed in the first leaves of seedlings cultivated in vitro. o The basal leaves of axenic plants of 2635-MM mutant show a slight curvature and no lobing. Bar = 1 cm