Fig. 1From: Identification of transcription factors of nitrate reductase gene promoters and NRE2 cis-element through yeast one-hybrid screening in Nicotiana tabacumCloning of NIA1 and NIA2 promoters and GUS expression levels. Electrophoretic diagram of cloned NIA1 promoter and NIA2 promoter (a). M: DNA marker; P-NIA1: the amplified fragment of NIA1 gene promoter; P-NIA2: the amplified fragment of the NIA2 gene promoter. Sequencing results of cloned (i) NIA1 promoter and (ii) NIA2 promoter (b). GUS histochemical staining results of tobacco leaves transformed by different promoter sequences (c) and the GUS content of tobacco leaves transformed by different promoter sequences measured by quantitative assay (d). * represents P < 0.05, and ** represents P < 0.01 when compared with normal NIA1 promoters; # represents P < 0.05 when compared with normal NIA2 promotersBack to article page