Fig. 9From: Transcriptomic profiling identifies differentially expressed genes associated with programmed cell death of nucellar cells in Ginkgo biloba L.Subcellular localization of calcium within the nucellar cells involved in pollen chamber formation. (a) At the early stage of ovule development, a relatively higher Ca2+ precipitation is found in both of vacuoles and nucleus, compared with that in the cytoplasm. (b) A negative control of (a) without Ca2+ particles. (c), (d), (f), and (g) The nucellar cells at the micropylar end become elongated in shape, and distribution of Ca2+ precipitation is found to be increased in these nucellar cells. (d) Magnified view of the circled area in (c) shows numerous Ca2+ particles within the vacuole and cytoplasm. (e) A negative control of (c) without Ca2+ particles. (g) Deformed endoplasmic reticula are enclosed within the vacuole. (h) Few of Ca2+ particles are distributed along the cytoplasm debris, with no detectable aggregation of Ca2+ in the nucleus of the dying nucellar cell. Arrows indicate Ca2+ particles. Bars in (a), (b), (c), (e), and (h) = 2 μm, and in (d), (f), and (g) = 0.2 μm. CW, cell wall; N, nucleus; V, vacuoleBack to article page