Fig. 1

Seed germination and root growth of wild type (WT), g6pd5, and g6pd6 mutant in response to ABA. a and b Seeds were germinated on 1/2 MS agar plates with 1 μM ABA. Photographs were taken 4 d after treatment. c 5-day-old seedlings were grown vertically on 1/2 MS agar plates supplemented with 10 μM ABA. Root growth was monitored and analyzed using ImageJ software. d Relative transcript levels of G6PD5 and G6PD6 in wild-type (Col-0) with different concentrations of ABA treatment. The transcript levels were normalized to Actin2. e The activities of G6PD in Arabidopsis WT and mutants exposed to ABA treatment. One-way Duncan’s test was performed, and statistically significant differences are indicated by different lower case letters (P < 0.05). Bar, 1 cm. The experiments were repeated at least three times with similar results, and data from one representative experiment are presented