Fig. 2
From: CRISPR/Cas9-Mediated SlNPR1 mutagenesis reduces tomato plant drought tolerance
CRISPR/Cas9-mediated genome editing. (a) Schematic illustration of the two target sites in SlNPR1 genomic sequence. Target 1 and target 2 sequences are shown in capital letters and the protospacer adjacent motif (PAM) sequence is marked in red. (b) Schematic diagram of pYLCRISPR/Cas9-SlNPR1 vector. HPT, hygromycin B phosphotransferase; Ubi, maize ubiquitin promoter; NLS, nuclear localization sequence; Tnos, gene terminator; AtU3d, Arabidopsis thaliana U3d promoter; AtU3b, A. thaliana U3b promoter. (c) CRISPR/Cas9-mediated efficient edit and variant genotypes of two target sequences in T0 plants.