Fig. 5

Expression analysis of Bna.AP1.A02 stop codon mutant lines. a Relative expression of Bna.AP1 putative downstream target genes b Relative expression of Bna.TFL1 paralogs. aa: Genotype carrying Bna.AP1.A02 mutant allele; AA: Genotype carrying Bna.AP1.A02 wildtype allele; Express617: Control. Expression levels of target genes were normalized against Bna.Actin total expression. For all genotypes tissue (SAM) sampling was done at BBCH30 between zeitgeber 8 h and 9 h. Three biological replicates and three technical replicates were used for each genotype. Error bars: standard error of the mean for biological replicates. The mean comparison between the genotypes for the investigated traits was performed by ANOVA test (P value = 0.0001), while the grouping was done using the LSD test (α ≤ 0.05) in R package ‘Agricolae’ version 1.2–8