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Table 1 Most representative clones obtained with each PK primer

From: PK-profiling method for identifying the expression of resistance-associated genes in partially resistant oats to crown rust

Primer Clones (accession no) Size (bp) BLASTx function (Brachypodium accession numbers)
PK1Fa PK1Fa114 (JZ978700) 180 Multidrug and toxin extrusion transporter protein (MATE) (XP_010233738)
PK1Fa113 (JZ978701) 159 Peptidase protein (XP_00357982)
PK1Fa115 (JZ978702) 150 LRR receptor-Mixed protein kinase (XP_014758670)
PK1Fa123 (JZ978703) 67 Hypersensitive response protein (XP_003557236)
PK1Fa2312 (JZ978704) 250 Receptor-like serine/threonine-protein kinase (XP_003569123)
PK1Fa2313 (JZ978705) 137 No significant similarity found
PK1Fa2315 (JZ978706) 159 Peptidase protein (XP_003579821)
PK1Fa2321 (JZ978707) 251 Receptor-like serine/threonine-protein kinase (XP_003569123)
PK1Fa2324 (JZ978708) 261 ADP-ribosylation factor (XP_003570479)
PK1Fb PK1Fb111 (JZ978709) 478 Cysteine-rich receptor-like protein Mixed kinase (XP_003562979)
PK1Fb113 (JZ978710) 256 Cysteine-rich receptor-like protein Mixed kinase (XP_003562978)
PK1Fb212 (JZ978711) 343 Cysteine-rich receptor-like protein Mixed kinase XP_010237455)
PK1Fb321 (JZ978712) 150 LRR receptor-Mixed protein kinase (XP_014758670)
PK3Fa PK3Fa14 (JZ978713) 269 Pto-like interacting Mixed protein kinase (XP_003568986)
PK3Fa23 (JZ978714) 227 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (XP_003574540)
PK3Fb PK3Fb11 (JZ978713) 269 Pto-like interacting Mixed protein kinase (XP_003568986)
PK3Fb12 (JZ978715) 224 Calcium-dependent Serine/threonine protein kinase (XP_003580397)
PK3Fb21 (JZ978714) 227 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (XP_003574540)
PK3Fb25 (JZ978716) 106 Serotonin N-acetyl protein transferase (XP_020178557.1)
PK4R1a PK4R1a11 (JZ978717) 223 LRR receptor-like serine/threonine-protein kinase (XP_003563758)
PK4R1a13 (JZ978718) 213 LRR receptor-like Mixed protein kinase (XP_003568447)
PK4R1a14 (JZ978719) 220 LRR receptor-like Mixed protein kinase (XP_003568447)
PK4R1a15 (JZ978720) 186 Chaperone protein (XP_003569728)
PK4R1a24 (JZ978721) 132 SAGA factor (XP_003576694)
PK4R1b PK4R1b11 (JZ978722) 256 Serine/threonine-protein kinase (XP_003566547)
PK4R1b15 (JZ978723) 184 Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) (XP_003576920)
PK4R1b21 (JZ978724) 430 Receptor serine/threonine kinase-like protein Xa21 (XP_010236334)
PK4R1b22 (JZ978725) 400 LRR Mixed kinase SERK (XP_003577373)
PK4R1b32 (JZ978726) 196 Alanyl-tRNA-synthetase (XP_003571715)
  1. No redundant clones obtained with each PK primer, GenBank accession numbers, sizes of the cloned fragments and expected functions for the most similar protein sequences of Brachypodium distachyon accessions searched by BLASTx