Fig. 4

Alignment with ClustalW2 of the amino acid sequences of the serine/threonine kinases of Brachypodium (black) with the highest similarity to those obtained in the PK-profiling (red). Black boxes indicate the 12 conserved subdomains of the protein kinases, with the exception of the subdomain X that are poorly conserved. Red boxes show the sequences cloned in this work. Asterisk points to the characteristic sequence of the active center (HRD). The colors of the amino acid refer to the degree of similarity: Black: Not similar; Yellow: Identical; Green: Similar; Blue: Conserved