Fig. 5
Relative expression levels of pathogenesis-related genes in transformed and F1 hybrid N. benthamiana. Leaves were sampled from transformed and F1 hybrid N. benthamiana to extract total RNA for RT-qPCR analysis. Non-transformed and transformed (T3) N. benthamiana were used as control for relative quantification of gene expression. EF-1α was used as internal control. a Relative expression of PR-genes in ptHR941 transformed N. benthamiana compared with non-transformed N. benthamiana control, b Relative expression of PR-genes in ptHR941-F1 hybrid compared with transformed (T3) N. benthamiana control, c Relative expression of PR-genes in ptHR375 transformed N. benthamiana compared with non-transformed N. benthamiana control, d Relative expression of PR-genes in ptHR375-F1 hybrid compared with transformed (T3) N. benthamiana control, and e Relative expression of ptHR genes in F1 hybrid compared with transformed (T3) N. benthamiana control. Results are the mean values from three independent experiments. Vertical bars indicate SD. Significance was determined by t-test: *P < 0.05, **P < 0.01