Fig. 2
Expression characteristics of ZmGPDH1 gene. ZmGPDH1 promoter-GUS assay in various tissues, including (a) rosette leaf, (b) stem, (c) cauline leaf, (d) mature silique, (e) flower, (f) flower bud, (g) stigma, (h) root, (i) 4-day-old plant, (j) 7-day-old plant, (k) 14-day-old plant and (l) immature silique. (m) The transcript level of GUS gene in different tissues of 5-week-old proZmGPDH1::GUS transgenic plants (T3 generation), including stems (ST), rosette leaves (RL), cauline leaves (CL), roots (RT), flowers (FL), flower buds (FLB), siliques (SL). The expression of GUS gene in SL was used as a calibrator. The transcriptional response of ZmGPDH1 in maize roots exposed to (n) NaCl or (o) mannitol treatments. The expression of ZmGPDH1 in untreated samples (control) harvested at each time point was used as a calibrator. (p) The analysis of GUS activity in response to mannitol or NaCl treatment. 7-day-old proZmGPDH1::GUS transgenic seedlings were transferred to either half-strength MS plates (1/2 MS), plates with 300 mM mannitol or plates with 150 mM NaCl for 12 h before GUS staining. Non-transformed wild-type (WT) was used as a control. Bars = 100 μm. The asterisks represented a significant difference as determined by the Student′s t-test (*P < 0.05, ** P < 0.01)